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EDC (12 Citations)




EDC is a heterobifunctional, water-soluble, zero-length carbodiimide crosslinker that is used to couple carboxyl groups to primary amines. EDC activates carboxyl groups first and forms amine reactive O-acylisourea imtermediate that spontaneously reacts with primary amines to form an amide bond and isourea by-product.

 

The unstable nature of the intermediate in aqueous solutions makes 2-step coupling, however in conjunction with N-hydroxysuccinimide, a 2-step coupling is possible.

 

EDC is ideal for peptide immobilization and hapten-carrier protein conjugation.

 

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Features

  • EDC mediated coupling efficiency improved by N-hydroxysuccinimide
  • Allows for 2-step coupling process
  • Water soluble
  • CAS Number: 25952-53-8
  • Molecular Formula: C8H17N3•HCl
  • Molecular Weight: 191.71 g/mol
  • Spacer Arm (Å): 0
  • Reactive Group: Carbodiimide
  • Reactive Toward: Primary Amine + Carboxyl
  • Membrane Permeable: NO
  • Water Soluble: YES
  • Cleavable/Reversible: NO
  • Reaction pH: 4.7 to 6.0

 


Applications

  • Couple haptens or peptides to carrier proteins
  • Conjugate carboxyl to amine groups in peptides and proteins 
  • Cross link proteins to carboxyl surfaces and beads
  • Convert carboxyls to amine-reactive Sulfo-NHS esters
  • Activate nanoparticles to amine-reactive Sulfo-NHS esters
  • DNA Labeling through 5' phosphate groups

 

Protocol
BC25-1
BC25-5
BC25-25
BC25-50
Material Safety Data Sheet
BC25-1
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BC25-5
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
BC25-25
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
BC25-50
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
Technical Literature
Biochemical Conjugation, Modification and Labeling of Oligonucleotide 5’ Phosphate Groups with EDC Protocols and tips for coupling with the EDC crosslinker
Protein Cross-Linking Handbook  A detailed guide to protein cross-linkers and their reactions.
Certificate Of Analysis
BC25-1
212409
BC25-5
191101
BC25-25
191101
BC25-50
212801

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