4-Nitrophenyl-beta-D-glucuronic acid

4-Nitrophenyl-beta-D-glucuronic acid
PNPG (Gus);
4-Nitrophenyl-β-D-glucuronic acid;
p-Nitrophenyl-β-D-glucuronide;
PNP-β-D-GlcA
 
MOLECULAR BIOLOGY GRADE
 
Slightly off-white to yellow crystalline powder.
 
PNPG (Gus) is a chromogenic β-glucuronidase substrate yielding a yellow solution upon cleavage. It is used to detect E. coli contamination in food and drinking water due to their β-glucuronidase activity.
 
Store dessicated at -20°C, protect from light.
 


Features

  • CAS#: 10344-94-2
  • Molecular Formula: C12H13NO9
  • Molecular Weight: 315.240 g/mol

 

 

 

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Material Safety Data Sheet
RC-458
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RC-461
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RC-462
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1. M. Kilian, P. BŸlow, Rapid diagnosis of Enterobacteriacea e. I. Detection of bacterial glycosidases. Acta Pathol. Microbiol. Scand. Sect. B 1976, 84, 245.
2. L. Le Minor, Te trathionate reductase, beta-glucuronidase, and ONPG-test in the genus Salmonella. Zbl. Bakt. Hyg. I. Abt. Orig. A 1 979, 243, 321.
3. M. Kilian, P. BŸlow, Rapid identification of Enterobacteriaceae. II. Use of a beta-glucuronidase detecting agar medium (PGUA agar) for the identification of E. coli in primary cultures of urine samples. Acta. Pat h. Microbiol. Scand. Sect. B 1979, 87, 271.
4. M. Manafi, et al., Fluorogenic and chromogenic substrates used in bacteri al diagnostics. Microbiol. Rev. 1991, 55, 335. 
5. I.D. Ogden, A.J. Watt, An evaluation of fluorogenic and chromogen ic assays for the direct enumeration of Escherichia col i. Lett. Appl. Microbiol. 1991, 13, 212.

6. R.H. Nimmo-Smith , p-Nitrophenyl-beta-glucuronide as substrate for beta-glucu ronidase. Biochim. Biophys. Acta 1961, 50, 166. 

Catalog Description Size Price(USD) Qty
RC-458
4-Nitrophenyl-beta-D-glucuronic acid 10 G
$711.00
RC-461
4-Nitrophenyl-beta-D-glucuronic acid 25 G
$1,615.00
RC-462
4-Nitrophenyl-beta-D-glucuronic acid 50 G
$2,953.00