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G-Sep™ Ni NTA Agarose Fast Flow

Catalog Description Size Price(USD) Qty
786-1040
G-Sep™ Ni NTA Agarose Fast Flow 5ml
$73.00
786-1041
G-Sep™ Ni NTA Agarose Fast Flow 25ml
$296.00
786-1042
G-Sep™ Ni NTA Agarose Fast Flow 100ml
$992.00
786-1043
G-Sep™ Ni NTA Agarose Fast Flow 500ml
$4,295.00

G-Sep™ Ni NTA Agarose Fast Flow

Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals.  G-Sep™NI NTA Agarose Fast Flow is specifically designed for the purification of recombinant proteins fused to the 6x histidine (6XHis) tag.  
 
 
The resin is specifically designed for the purification of recombinant proteins fused to the 6X histidine (6XHis) tag expressed in bacteria, insects, and mammalian cells.  The resin is high affinity and selectivity for recombinant fusion proteins that are tagged with six tandem histidine residues.  
 
 
G-Sep™ NI NTA Agarose Fast Flow (FF) resin is nickel ions immobilized onto highly cross-linked 6% agarose beads. The G-Sep™ NTA Agarose Fast Flow (FF) resins have high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols. G-Sep™ Ni NTA Agarose Fast Flow (FF) resin using cobalt is also available

 

Features

  • Matrix: Cross-linked agarose beads, 6%
  • Bead form: Spherical, diameter 50-160μm
  • Spacer: Epichlorohydrin
  • Chelating Agent: nitrilotriacetic acid (NTA)
  • pH stability Working Range: 3-12
  • pH stability Cleaning-in-Place (CIP): 2-14
  • Maximum Flow Velocity: 450cm/h
  • Exclusion limit(globular proteins): 4 x 106
  • Physical Stability: Negligible volume variation due to changes in pH or ionic strength
  • Chemical Stability: Stable to all commonly used aqueous buffers: 6M urea, 8M guanidine hydrochloride,
  • Autoclavable: 121°C, pH 7, for 30 min
  • Storage Conditions: 2 to 8°C, 20% Ethanol
Protocol
786-1040
786-1041
786-1042
786-1043
Material Safety Data Sheet
786-1040
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786-1041
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-1042
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-1043
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
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