Datasheet |
|
Target Protein |
14-3-3 θ/τ (Ser232) |
Clonality |
Polyclonal |
Storage/Stability |
-20°C/1 year |
Application |
WB,IHC-p,IF,ELISA |
Recommended Dilution |
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
Concentration |
1 mg/ml |
Reactive Species |
Human,Mouse,Rat |
Host Species |
Rabbit |
Immunogen |
Synthesized phospho-peptide around the phosphorylation site of human 14-3-3 θ/τ (phospho Ser232) |
Specificity |
Phospho-14-3-3 θ/τ (S232) Polyclonal Antibody detects endogenous levels of 14-3-3 θ/τ protein only when phosphorylated at S232. |
Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
Form |
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Gene Name |
YWHAQ |
Accession No. |
P27348 P68254 P68255 |
Alternate Names |
YWHAQ; 14-3-3 protein theta; 14-3-3 protein T-cell; 14-3-3 protein tau; Protein HS1 |
Description |
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta(YWHAQ) Homo sapiens This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq, Jul 2008], |
Cell Pathway/ Category |
Cell_Cycle_G1S,Cell_Cycle_G2M_DNA,Oocyte meiosis,Neurotrophin,Pathogenic Escherichia coli infection, |
Protein Expression |
B-cell lymphoma,Brain,Eye,H |
Subcellular Localization |
cytoplasm,mitochondrion,cytosol,focal adhesion,membrane,cytoplasmic vesicle membrane,protein complex,extracellular exosome, |
Protein Function |
function:Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.,similarity:Belongs to the 14-3-3 family.,subcellular location:In neurons, axonally transported to the nerve terminals.,subunit:Homodimer. Interacts with PCTK1 (By similarity). Interacts with SSH1. Interacts with CDKN1B ('Thr-198' phosphorylated form); the interaction translocates CDKN1B to the cytoplasm.,tissue specificity:Abundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement., |
Usage |
For Research Use Only! Not for diagnostic or therapeutic procedures. |