Senescence Histochemical Staining Kit (2 Citations)
• X-Gal conveniently aliquoted into 4 separate vials to prolong shelf-life!
• 4 x 100 assays!
• 200ml assay volume!
• Works on adhered/suspended cells or frozen tissue sections!
Senescence kit is designed to be more convenient and cost efficient than other kits on the market. Our senescence cells histochemical staining kit has been optimized to stain Sa-β-Gal specifically at pH 6 while remaining user friendly. Our high quality X-Gal substrate comes conveniently aliquoted in 4 vials to extend shelf-life and ensure high reproducibility from your first to last experiment.
Senescence associated β-Galactosidase activity at pH 6 is one of the most frequently used biomarkers to detect senescent cells. This enzyme’s activity at this specific pH is only observed in senescent cells due to the overexpression of endosomal β-Galactosidase. While there are several modern substrates that are more sensitive, X-Gal remains the most popular substrate to detect Sa-β-Gal. This is due to the simplicity of the assay as well as being one of the most established methods of detecting senescence and therefore less likely to raise questions among reviewers.
- "Pambianchi, Erika et al (2021) Alaskan Bog Blueberry (Vaccinium uliginosu) Extract as an Innovative Topical Approach to Prevent UV-Induced Skin Damage. COSMETICS. https://doi.org/10.3390/cosmetics 8040112"
- Das, Monalisa et al (2021) Comparative evaluation of proliferative potential and replicative senescence associated changes in mesenchymal stem cells derived from dental pulp and umbilical cord. CELL TISSUE BANK. https://doi.org/10.1007/s10561-021-09926-8