CytoScan™-fluoro Cytotoxicity Assay

CytoScan™-fluoro Cytotoxicity Assay

The CytoScan™-fluoro is based on the quantification of cellular lactate dehydrogenase (LDH) released when cells are damaged or under stress. Lactate dehydrogenase released into the culture medium is measured with a diaphorase coupled enzymatic assay that results in the conversion of a non-fluorescent compound (resazurin) to a fluorescent compound (resorufin), which is measured using a fluorometer. The assay detects even low level damage to cell membrane not detected with other methods.

The CytoScan™-fluoro kit does not damage healthy cells and the assay can be performed directly in the cell culture wells containing a mixed population of viable and damaged cells. The kit is supplied with substrate mix, assay buffer, and stop solution.


 


Features

  • Quantitatively measures LDH release
  • Fluorometric assay
  • Assays performed directly in cell culture wells

 


Applications

  • For the detection of cell toxicity, death, viability or proliferation.
  • Ideal for high throughput screening.

 

 

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Protocol
786-211
Material Safety Data Sheet
786-211
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Technical Literature
Bioassays Handbook
Protein Assay Handbook & Selection Guide  An introduction to protein assays.
Catalog Description Size Price(USD) Qty
786-211
CytoScan™-fluoro Cytotoxicity Assay 500 Assays
$311.00