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Transformation of E. coli with p-Amylase (Lab 8C)

This kit is designed to teach 8 groups of four students the principles of bacterial transformation.

 

The kit contains the reagents to allow students to:

  • Generate E.coli comptent cells
  • Transform competent cells with the p-Amylase plasmid
  • Grow transformed colonies on selection plates of LB/starch/amp agar plates
  • Understand antibiotic selectivity
  • Visualize the new bacterial phenotype (amylase production) on the selection plates

 


Features

 

  • For 8 groups of 4 students 
  • Requires waterbath and incubation oven, micropipettes and tips

 


Applications

  • HS-LS1: From Molecules to Organisms: Structures and Processes
  • LS1.A: Structure and Function

 

 

Protocol
BTNM-8C
Material Safety Data Sheet
BTNM-8C
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