IBS™ Buffer

IBS™ Buffer
The expression of recombinant proteins is a popular and routinely used technique in protein studies.  The expression of recombinant proteins often has one drawback and that is the recombinant proteins aggregate and form inclusion bodies, especially when expressed at high levels.  The aggregated proteins are difficult to solubilize, due to the nature of aggregates, however we offer a selection of products for dealing with the range of issues involved with solubilizing and recovering active proteins from inclusion bodies.

The IBS™ buffer is specifically developed for solubilization of inclusion bodies. 
Simple to use protocol as inclusion bodies are suspended in IBS™ Buffer, where they readily dissolve releasing the proteins of interest.  Once the inclusion bodies are solubilized, the sample is ready for further analysis and other downstream applications.
IBS™ Buffer system is compatible with widely used protein folding protocols, with NI™ Protein Assay (Cat. # 786-005) and running SDS-PAGE analysis using PAGE-Perfect™ kit
(Cat. # 786-123).
The use of IBS™ Buffer also facilitates the use of PROTEIN-Foldase™ (Cat. # 786-185), a kit for optimizing protein folding procedures.

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Material Safety Data Sheet
Technical Literature
Mass Spectrometry Sample Prep Handbook A guide to the preparation of protein samples for Mass Spectrometry, including protein extraction, clean-up and peptide generation.
Plant Proteomics Handbook
Protease & Phosphatase Inhibitors & Proteases Handbook  A handbook & selection guide for inhibitors of protease & phosphatases & for proteases & assays
Protein Electrophoresis Handbook A guide to 1D and 2D protein electrophoresis products, including protein markers, electrophoresis buffers, 2D electrophoresis reagents, clean-up reagents and stains. The guide also offers protein sample preparation products.
Sample Preparation Handbook For Lysis Buffers, Fractionation, Dialysis, Protein Concentration and Enrichment
Western Blotting Handbook From protein electrophoresis and Western transfer to blocking, probing and detection of membrane immobilized proteins.
Catalog Description Size Price(USD) Qty
IBS™ Buffer 100ml