ITC0053
ITC0053
ITC0053
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- Catalog: ITC0053
- Gene/Protein: F13A1
- Product Description: Immunotag™ Cleaved-Factor XIIIa (G39) Polyclonal Antibody
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Description
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Specifications
Antibody Specification Datasheet 
IMPORTANT NOTE This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. Target Protein Factor XIIIa (G39) Clonality Polyclonal Storage/Stability -20°C/1 year Application WB,ELISA Recommended Dilution Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. Concentration 1 mg/ml Reactive Species Human Host Species Rabbit Immunogen The antiserum was produced against synthesized peptide derived from human FA13A. AA range:20-69 Specificity Cleaved-Factor XIIIa (G39) Polyclonal Antibody detects endogenous levels of fragment of activated Factor XIIIa protein resulting from cleavage adjacent to G39. Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen Form Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Gene Name F13A1 Accession No. P00488 Q8BH61 Alternate Names F13A1; F13A; Coagulation factor XIII A chain; Coagulation factor XIIIa; Protein-glutamine gamma-glutamyltransferase A chain; Transglutaminase A chain Description coagulation factor XIII A chain(F13A1) Homo sapiens This gene encodes the coagulation factor XIII A subunit. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as plasma carrier molecules. Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin. Upon cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. It also crosslinks alpha-2-plasmin inhibitor, or Cell Pathway/ Category Complement and coagulation cascades, Protein Expression Brain,Pancreas,Plasma, Subcellular Localization extracellular region,platelet alpha granule lumen,blood microparticle, Protein Function catalytic activity:Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH(3).,cofactor:Binds 1 calcium ion per subunit.,disease:Defects in F13A1 are the cause of F13A deficiency [MIM:134570]. F13A deficiency is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women. In addition to the common presentation such as subcutaneous and intramuscular haematomas, severe bleeding such as intracranial hemorrhages may occur.,function:Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.,online information:Factor XIII entry,online information:The Singapore human mutation and polymorphism database,polymorphism:There are four main allelic forms of this protein; F13A*1A, F13A*1B, F13A*2A and F13A*2B. In addition two other intermediate forms (F13A*(2)A and F13A*(2)B) seem to exist. The sequence shown is that of F13A*(2)B.,PTM:The activation peptide is released by thrombin.,similarity:Belongs to the transglutaminase superfamily. Transglutaminase family.,subcellular location:Secreted into the blood plasma. Cytoplasmic in most tissues, but also secreted in the blood plasma.,subunit:Tetramer of two A chains and two B chains., Usage For Research Use Only! Not for diagnostic or therapeutic procedures. - Reviews
| Antibody Specification | |
| Datasheet | |
| IMPORTANT NOTE | This product is custom manufactured with a lead time of 3-4 weeks. Once in production, this item cannot be cancelled from an order and is not eligible for return. |
| Target Protein | Factor XIIIa (G39) |
| Clonality | Polyclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,ELISA |
| Recommended Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human |
| Host Species | Rabbit |
| Immunogen | The antiserum was produced against synthesized peptide derived from human FA13A. AA range:20-69 |
| Specificity | Cleaved-Factor XIIIa (G39) Polyclonal Antibody detects endogenous levels of fragment of activated Factor XIIIa protein resulting from cleavage adjacent to G39. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Gene Name | F13A1 |
| Accession No. | P00488 Q8BH61 |
| Alternate Names | F13A1; F13A; Coagulation factor XIII A chain; Coagulation factor XIIIa; Protein-glutamine gamma-glutamyltransferase A chain; Transglutaminase A chain |
| Description | coagulation factor XIII A chain(F13A1) Homo sapiens This gene encodes the coagulation factor XIII A subunit. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as plasma carrier molecules. Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin. Upon cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. It also crosslinks alpha-2-plasmin inhibitor, or |
| Cell Pathway/ Category | Complement and coagulation cascades, |
| Protein Expression | Brain,Pancreas,Plasma, |
| Subcellular Localization | extracellular region,platelet alpha granule lumen,blood microparticle, |
| Protein Function | catalytic activity:Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH(3).,cofactor:Binds 1 calcium ion per subunit.,disease:Defects in F13A1 are the cause of F13A deficiency [MIM:134570]. F13A deficiency is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women. In addition to the common presentation such as subcutaneous and intramuscular haematomas, severe bleeding such as intracranial hemorrhages may occur.,function:Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.,online information:Factor XIII entry,online information:The Singapore human mutation and polymorphism database,polymorphism:There are four main allelic forms of this protein; F13A*1A, F13A*1B, F13A*2A and F13A*2B. In addition two other intermediate forms (F13A*(2)A and F13A*(2)B) seem to exist. The sequence shown is that of F13A*(2)B.,PTM:The activation peptide is released by thrombin.,similarity:Belongs to the transglutaminase superfamily. Transglutaminase family.,subcellular location:Secreted into the blood plasma. Cytoplasmic in most tissues, but also secreted in the blood plasma.,subunit:Tetramer of two A chains and two B chains., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |