ITC0096
ITC0096
- Catalog: ITC0096
- Gene/Protein: KNG1
- Product Description: Immunotag™ Cleaved-Kininogen-1 HC (K380) Polyclonal Antibody
460.0000
Price in reward points: 460
| Antibody Specification | |
| Datasheet | 
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| Target Protein | Kininogen-1 HC (K380) |
| Clonality | Polyclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,ELISA |
| Recommended Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human |
| Host Species | Rabbit |
| Immunogen | Synthesized peptide derived from Cleaved-Kininogen-1 HC (K380) . at AA range: 300-380 |
| Specificity | Cleaved-Kininogen-1 HC (K380) Polyclonal Antibody detects endogenous levels of fragment of activated Kininogen-1 HC protein resulting from cleavage adjacent to K380. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Gene Name | KNG1 |
| Accession No. | P01042 O08677 |
| Alternate Names | KNG1; BDK; KNG; Kininogen-1; Alpha-2-thiol proteinase inhibitor; Fitzgerald factor; High molecular weight kininogen; HMWK; Williams-Fitzgerald-Flaujeac factor |
| Description | kininogen 1(KNG1) Homo sapiens This gene uses alternative splicing to generate two different proteins- high molecular weight kininogen (HMWK) and low molecular weight kininogen (LMWK). HMWK is essential for blood coagulation and assembly of the kallikrein-kinin system. Also, bradykinin, a peptide causing numerous physiological effects, is released from HMWK. Bradykinin also functions as an antimicrobial peptide with antibacterial and antifungal activity. In contrast to HMWK, LMWK is not involved in blood coagulation. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Nov 2014], |
| Cell Pathway/ Category | Complement and coagulation cascades, |
| Protein Expression | Ascites,Kidney,Liver,Plasma, |
| Subcellular Localization | extracellular region,extracellular space,plasma membrane,platelet alpha granule lumen,extracellular exosome,blood microparticle, |
| Protein Function | disease:Defects in KNG1 are the cause of high molecular weight kininogen deficiency (HMWK deficiency) [MIM:228960]. HMWK deficiency is an autosomal recessive coagulation defect. Patients with HWMK deficiency do not have a hemorrhagic tendency, but they exhibit abnormal surface-mediated activation of fibrinolysis.,function:(1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.,online information:High molecular weight kininogen entry,polymorphism:The T-kinin peptide is missing residues 378 to 380, probably as a result of a naturally occurring variant. The complete sequence of the T-kinin peptide is therefore ISRPPGFSPFR. This peptide is associated with malignant tumors but not with benign ones.,PTM:Bradykinin is released from kininogen by plasma kallikrein.,PTM:Hydroxylation of Pro-383 occurs prior to the release of bradykinin.,similarity:Contains 3 cystatin domains.,tissue specificity:Plasma. T-kinin is detected in malignant ovarian, colon and breast carcinomas, but not in benign tumors., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |
| Material Safety Data Sheet | |
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