ITM0036
ITM0036
- Catalog: ITM0036
- Gene/Protein: APOB
- Product Description: Immunotag™ ApoB Monoclonal Antibody
504.0000
Price in reward points: 504
| Antibody Specification | |
| Datasheet | 
|
| Target Protein | ApoB |
| Clonality | Monoclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,IF,FCM,ELISA |
| Recommended Dilution | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. Flow cytometry: 1/200 - 1/400. ELISA: 1/10000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human |
| Host Species | Mouse |
| Immunogen | Purified recombinant fragment of human ApoB expressed in E. Coli. |
| Specificity | ApoB Monoclonal Antibody detects endogenous levels of ApoB protein. |
| Purification | Affinity purification |
| Form | Ascitic fluid containing 0.03% sodium azide. |
| Gene Name | APOB |
| Accession No. | P04114 |
| Alternate Names | APOB; Apolipoprotein B-100; Apo B-100 |
| Description | apolipoprotein B(APOB) Homo sapiens This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Jul 2008], |
| Protein Expression | Brain,Epithelium,Liver,Plasma,Small intestine, |
| Subcellular Localization | extracellular region,extracellular space,cytoplasm,early endosome,endoplasmic reticulum lumen,endoplasmic reticulum membrane,Golgi apparatus,cytosol,plasma membrane,endosome membrane,actin cytoskeleton,clathrin-coated endocytic vesicle |
| Protein Function | disease:Defects in APOB are a cause of familial hypobetalipoproteinemia (FHBL) [MIM:107730]. FHBL is a genetically heterogeneous autosomal co-dominant disorder, associated with reduced plasma concentrations of apoB, LDL and VLDL. Heterozygotes for FHBL are usually asymptomatic with LDL cholesterol and apoB-100 concentrations less than 50% of those in normal plasma. Homozygotes have extremely low plasma LDL cholesterol and apoB-100 concentrations, and clinical presentation may vary from no symptoms to severe gastrointestinal and neurological dysfunction similar to abetalipoproteinemia [MIM:200100].,disease:Defects in APOB are a cause of familial ligand-defective apolipoprotein B-100 (FDB) [MIM:144010]. FDB is a dominantly inherited disorder of lipoprotein metabolism leading to hypercholesterolemia and increased proneness to coronary artery disease (CAD). The plasma cholesterol levels are dramatically elevated due to impaired clearance of LDL particles by defective APOB/E receptors.,disease:Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.,function:Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.,online information:Apolipoprotein B entry,online information:The Singapore human mutation and polymorphism database,PTM:Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle.,RNA editing:The stop codon (UAA) at position 2180 is created by RNA editing. Apo B-48, derived from the fully edited RNA, is produced only in the intestine and is found in chylomicrons. Apo B-48 is a shortened form of apo B-100 which lacks the LDL-receptor region. The unedited version (apo B-100) is produced by the liver and is found in the VLDL and LDL.,similarity:Contains 1 vitellogenin domain., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |
| Material Safety Data Sheet | |
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