ITM0143
ITM0143
- Catalog: ITM0143
- Gene/Protein: CDC27
- Product Description: Immunotag™ CDC27 Monoclonal Antibody
504.0000
Price in reward points: 504
| Antibody Specification | |
| Datasheet | 
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| Target Protein | CDC27 |
| Clonality | Monoclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,IHC-p,ELISA |
| Recommended Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human |
| Host Species | Mouse |
| Immunogen | Purified recombinant fragment of human CDC27 expressed in E. Coli. |
| Specificity | CDC27 Monoclonal Antibody detects endogenous levels of CDC27 protein. |
| Purification | Affinity purification |
| Form | Ascitic fluid containing 0.03% sodium azide. |
| Gene Name | CDC27 |
| Accession No. | P30260 A2A6Q5 |
| Alternate Names | CDC27; ANAPC3; D0S1430E; D17S978E; Cell division cycle protein 27 homolog; Anaphase-promoting complex subunit 3; APC3; CDC27 homolog; CDC27Hs; H-NUC |
| Description | cell division cycle 27(CDC27) Homo sapiens The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been i |
| Cell Pathway/ Category | Cell_Cycle_G1S,Cell_Cycle_G2M_DNA,Oocyte meiosis,Ubiquitin mediated proteolysis,Progesterone-mediated oocyte maturation, |
| Protein Expression | Epithelium,Uterus, |
| Subcellular Localization | nucleus,nucleoplasm,anaphase-promoting complex,cytoplasm,centrosome,spindle,cytosol,spindle microtubule,integral component of membrane, |
| Protein Function | PTM:Phosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis.,similarity:Belongs to the APC3/CDC27 family.,similarity:Contains 9 TPR repeats.,subunit:Interacts with RB., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |
| Material Safety Data Sheet | |
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