ITM0253
ITM0253
- Catalog: ITM0253
- Gene/Protein: SETDB1
- Product Description: Immunotag™ ESET Monoclonal Antibody
504.0000
Price in reward points: 504
| Antibody Specification | |
| Datasheet | 
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| Target Protein | ESET |
| Clonality | Monoclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,ELISA |
| Recommended Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Mouse,Monkey |
| Host Species | Mouse |
| Immunogen | Purified recombinant fragment of human ESET expressed in E. Coli. |
| Specificity | ESET Monoclonal Antibody detects endogenous levels of ESET protein. |
| Purification | Affinity purification |
| Form | Ascitic fluid containing 0.03% sodium azide. |
| Gene Name | SETDB1 |
| Accession No. | Q15047 O88974 |
| Alternate Names | SETDB1; KIAA0067; KMT1E; Histone-lysine N-methyltransferase SETDB1; ERG-associated protein with SET domain; ESET; Histone H3-K9 methyltransferase 4; H3-K9-HMTase 4; Lysine N-methyltransferase 1E; SET domain bifurcated 1 |
| Description | SET domain bifurcated 1(SETDB1) Homo sapiens This gene encodes a histone methyltransferase which regulates histone methylation, gene silencing, and transcriptional repression. This gene has been identified as a target for treatment in Huntington Disease, given that gene silencing and transcription dysfunction likely play a role in the disease pathogenesis. Alternatively spliced transcript variants of this gene have been described.[provided by RefSeq, Jun 2011], |
| Cell Pathway/ Category | Lysine degradation, |
| Protein Expression | Bone marrow,Hepatocyte,Muscle,Uterus, |
| Subcellular Localization | nucleus,nucleoplasm,chromosome,cytoplasm,plasma membrane,intracellular membrane-bounded organelle, |
| Protein Function | catalytic activity:S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N(6)-methyl-L-lysine.,domain:The pre-SET, SET and post-SET domains are all required for methyltransferase activity. The 347-amino-acid insertion in the SET domain has no effect on the catalytic activity.,function:Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins.,miscellaneous:Highly up-regulated in Huntington disease patients, suggesting that participates in the altered chromatin modulation and transcription dysfunction observed in Huntington disease. Its down-regulation has salubrious effects on patients, suggesting that it may be a promising treatment in Huntington disease patients.,miscellaneous:Isoform 2 lacks all domains required for histone methyltransferase activity.,similarity:Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.,similarity:Contains 1 MBD (methyl-CpG-binding) domain.,similarity:Contains 1 post-SET domain.,similarity:Contains 1 pre-SET domain.,similarity:Contains 1 SET domain.,similarity:Contains 2 Tudor domains.,subcellular location:Associated with non-pericentromeric regions of chromatin. Excluded from nucleoli and islands of condensed chromatin.,subunit:Interacts with MBD1; interaction is abolished when MBD1 is sumoylated. Interacts with ATF7IP and ATF7IP2; the interaction with ATF7IP is required to stimulate histone methyltransferase activity and facilitate the conversion of dimethylated to trimethylated H3 'Lys-9'. During DNA replication, it is recruited by SETDB1 to form a S phase-specific complex that facilitates methylation of H3 'Lys-9' during replication-coupled chromatin assembly and is at least composed of the CAF-1 subunit CHAF1A, MBD1 and SETDB1. Interacts with ERG, TRIM28/TIF1B, CBX1, CBX5, DNMT3A, HDAC1, HDAC2, SIN3A, SIN3B, DNMT3B and SUMO2.,tissue specificity:Widely expressed. High expression in testis., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |
| Material Safety Data Sheet | |
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