ITM0350
ITM0350
- Catalog: ITM0350
- Gene/Protein: HK2
- Product Description: Immunotag™ HXK II Monoclonal Antibody
504.0000
Price in reward points: 504
| Antibody Specification | |
| Datasheet | 
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| Target Protein | HXK II |
| Clonality | Monoclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,IHC-p,FCM,ELISA |
| Recommended Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/200 - 1/1000. Flow cytometry: 1/200 - 1/400. ELISA: 1/10000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human |
| Host Species | Mouse |
| Immunogen | Purified recombinant fragment of human HXK II expressed in E. Coli. |
| Specificity | HXK II Monoclonal Antibody detects endogenous levels of HXK II protein. |
| Purification | Affinity purification |
| Form | Ascitic fluid containing 0.03% sodium azide. |
| Gene Name | HK2 |
| Accession No. | P52789 O08528 |
| Alternate Names | HK2; Hexokinase-2; Hexokinase type II; HK II; Muscle form hexokinase |
| Description | hexokinase 2(HK2) Homo sapiens Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq, Apr 2009], |
| Cell Pathway/ Category | Glycolysis / Gluconeogenesis,Fructose and mannose metabolism,Galactose metabolism,Starch and sucrose metabolism,Amino sugar and nucleotide sugar metabolism,Insulin_Receptor,Type II diabetes mellitus, |
| Protein Expression | Blood,Endometrium carcinoma cell line,Muscle,Placenta,Skeletal muscle,Testis, |
| Subcellular Localization | cell,mitochondrial outer membrane,cytosol,membrane, |
| Protein Function | catalytic activity:ATP + D-hexose = ADP + D-hexose 6-phosphate.,domain:The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.,enzyme regulation:Hexokinase is an allosteric enzyme inhibited by its product Glc-6-P.,miscellaneous:In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).,online information:Hexokinase entry,pathway:Carbohydrate metabolism; hexose metabolism.,polymorphism:Although found in NIDDM patients, genetic variations of HK2 do not contribute to the disease.,similarity:Belongs to the hexokinase family.,subcellular location:Its hydrophobic N-terminal sequence may be involved in membrane binding.,subunit:Monomer.,tissue specificity:Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |
| Material Safety Data Sheet | |
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