ITM0381
ITM0381
- Catalog: ITM0381
- Gene/Protein: ERN1
- Product Description: Immunotag™ IRE1α Monoclonal Antibody
504.0000
Price in reward points: 504
| Antibody Specification | |
| Datasheet | 
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| Target Protein | IRE1α |
| Clonality | Monoclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,IHC-p,ELISA |
| Recommended Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human |
| Host Species | Mouse |
| Immunogen | Purified recombinant fragment of human IRE1α (aa282-433) expressed in E. Coli. |
| Specificity | IRE1α Monoclonal Antibody detects endogenous levels of IRE1α protein. |
| Purification | Affinity purification |
| Form | Ascitic fluid containing 0.03% sodium azide. |
| Gene Name | ERN1 |
| Accession No. | O75460 Q9EQY0 |
| Alternate Names | ERN1; IRE1; Serine/threonine-protein kinase/endoribonuclease IRE1; Endoplasmic reticulum-to-nucleus signaling 1; Inositol-requiring protein 1; hIRE1p; Ire1-alpha; IRE1a |
| Description | endoplasmic reticulum to nucleus signaling 1(ERN1) Homo sapiens The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. [provided by RefSeq, Jul 2008], |
| Cell Pathway/ Category | Alzheimer's disease, |
| Protein Expression | Brain,Endothelial cell,Leukocyte,Liver,Testis, |
| Subcellular Localization | nuclear inner membrane,cytoplasm,mitochondrion,endoplasmic reticulum,endoplasmic reticulum membrane,integral component of endoplasmic reticulum membrane,Ire1 complex,AIP1-IRE1 complex,IRE1-TRAF2-ASK1 complex,IRE1-RACK1-PP2A co |
| Protein Function | catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:The kinase domain is activated by trans-autophosphorylation. Kinase activity is required for activation of the endoribonuclease domain.,function:Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.,PTM:Autophosphorylated.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.,similarity:Contains 1 KEN domain.,similarity:Contains 1 protein kinase domain.,subunit:Homodimer; disulfide-linked. Dimer formation is driven by hydrophobic interactions within the N-terminal luminal domains and stabilized by disulfide bridges. Also binds HSPA5, a negative regulator of the unfolded protein response. This interaction may disrupt homodimerization and prevent activation of ERN1.,tissue specificity:Ubiquitously expressed. High levels observed in pancreatic tissue., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |
| Material Safety Data Sheet | |
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