ITM0416
ITM0416
- Catalog: ITM0416
- Gene/Protein: LIN28A
- Product Description: Immunotag™ LIN-28 Monoclonal Antibody
504.0000
Price in reward points: 504
| Antibody Specification | |
| Datasheet | 
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| Target Protein | LIN-28 |
| Clonality | Monoclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,IF,ELISA |
| Recommended Dilution | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human |
| Host Species | Mouse |
| Immunogen | Purified recombinant fragment of LIN-28 (aa93-209) expressed in E. Coli. |
| Specificity | LIN-28 Monoclonal Antibody detects endogenous levels of LIN-28 protein. |
| Purification | Affinity purification |
| Form | Antibody are purified by protein G affinity chromatography. Liquid in 0.01M Phosphate buffer, pH 7.4 containing 0.03% sodium azide. |
| Gene Name | LIN28A |
| Accession No. | Q9H9Z2 Q8K3Y3 |
| Alternate Names | LIN28A; CSDD1; LIN28; ZCCHC1; Protein lin-28 homolog A; Lin-28A; Zinc finger CCHC domain-containing protein 1 |
| Description | lin-28 homolog A(LIN28A) Homo sapiens This gene encodes a LIN-28 family RNA-binding protein that acts as a posttranscriptional regulator of genes involved in developmental timing and self-renewal in embryonic stem cells. The encoded protein functions through direct interaction with target mRNAs and by disrupting the maturation of certain miRNAs involved in embryonic development. This protein prevents the terminal processing of the LET7 family of microRNAs which are major regulators of cellular growth and differentiation. Aberrant expression of this gene is associated with cancer progression in multiple tissues. [provided by RefSeq, Sep 2015], |
| Protein Expression | Placenta,Teratocarcinoma, |
| Subcellular Localization | cytoplasmic mRNA processing body,nucleus,nucleolus,cytoplasm,rough endoplasmic reticulum,cytosol,cytoplasmic stress granule, |
| Protein Function | developmental stage:Expressed in fetal liver. Expression decreases during differentiation of ES cells or upon induction of neuronal differentiation by retinoic acid.,domain:The CSD domain is required for function in muscle differentiation.,function:Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression.,induction:Can be negatively regulated by the interaction of microRNAs miR-125a and miR-125b with at least two miRNA responsive elements (miREs) in the 3'-UTR of this gene. These interactions may reduce both translation efficiency and mRNA abundance. Negatively regulated by retinoic acid.,similarity:Belongs to the lin-28 family.,similarity:Contains 1 CSD (cold-shock) domain.,similarity:Contains 2 CCHC-type zinc fingers.,subcellular location:Nucleolar localization observed in 10 - 15% of the nuclei in differentiated myotubes.,subunit:During skeletal muscle differentiation, associated with translation initiation complexes in the polysomal compartment. Directly interacts with EIF3S2. Interaction with NCL is RNA-dependent.,tissue specificity:Expressed in embryonic stem cells (ES cells), placenta and testis., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |
| Material Safety Data Sheet | |
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