ITM0517
ITM0517
- Catalog: ITM0517
- Gene/Protein: EIF2AK3
- Product Description: Immunotag™ PERK Monoclonal Antibody
504.0000
Price in reward points: 504
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Antibody Specification | |
Datasheet | |
Target Protein | PERK |
Clonality | Monoclonal |
Storage/Stability | -20°C/1 year |
Application | WB,ELISA |
Recommended Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
Concentration | 1 mg/ml |
Reactive Species | Human |
Host Species | Mouse |
Immunogen | Purified recombinant fragment of human PERK expressed in E. Coli. |
Specificity | PERK Monoclonal Antibody detects endogenous levels of PERK protein. |
Purification | Affinity purification |
Form | Ascitic fluid containing 0.03% sodium azide. |
Gene Name | EIF2AK3 |
Accession No. | Q9NZJ5 Q9Z2B5 |
Alternate Names | EIF2AK3; PEK; PERK; Eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; HsPEK |
Description | eukaryotic translation initiation factor 2 alpha kinase 3(EIF2AK3) Homo sapiens The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015], |
Cell Pathway/ Category | Alzheimer's disease, |
Protein Expression | Brain,Fetal brain,Liver,Pancreas,Pooled,Tes |
Subcellular Localization | cytoplasm,endoplasmic reticulum,endoplasmic reticulum membrane,membrane,integral component of endoplasmic reticulum membrane,perinuclear region of cytoplasm, |
Protein Function | catalytic activity:ATP + a protein = ADP + a phosphoprotein.,disease:Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS) [MIM:226980]; also known as multiple epiphyseal dysplasia with early-onset diabetes mellitus. WRS is a rare autosomal recessive disorder, characterized by permanent neonatal or early infancy insulin-dependent diabetes and, at a later age, epiphyseal dysplasia, osteoporosis, growth retardation and other multisystem manifestations, such as hepatic and renal dysfunctions, mental retardation and cardiovascular abnormalities.,domain:The lumenal domain senses perturbations in protein folding in the ER, probably through reversible interaction with HSPA5/BIP.,enzyme regulation:Perturbation in protein folding in the endoplasmic reticulum (ER) promotes reversible dissociation from HSPA5/BIP and oligomerization, resulting in transautophosphorylation and kinase activity induction.,function:Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin D1.,induction:By ER stress.,PTM:Autophosphorylated.,PTM:N-glycosylated.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Forms dimers with HSPA5/BIP in resting cells. Oligomerizes in ER-stressed cells. Interacts with DNAJC3.,tissue specificity:Ubiquitous. A high level expression is seen in secretory tissues., |
Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |