ITM0566
ITM0566
- Catalog: ITM0566
- Gene/Protein: RSPO1
- Product Description: Immunotag™ R-Spondin Monoclonal Antibody
504.0000
Price in reward points: 504
| Antibody Specification | |
| Datasheet | 
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| Target Protein | R-Spondin |
| Clonality | Monoclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,ELISA |
| Recommended Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human |
| Host Species | Mouse |
| Immunogen | Purified recombinant fragment of R-spondin1 expressed in E. Coli. |
| Specificity | R-Spondin Monoclonal Antibody detects endogenous levels of R-Spondin protein. |
| Purification | Affinity purification |
| Form | Ascitic fluid containing 0.03% sodium azide. |
| Gene Name | RSPO1 |
| Accession No. | Q2MKA7 Q9Z132 |
| Alternate Names | RSPO1; R-spondin-1; Roof plate-specific spondin-1; hRspo1 |
| Description | R-spondin 1(RSPO1) Homo sapiens This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014], |
| Protein Expression | Uterus, |
| Subcellular Localization | extracellular region,nucleus, |
| Protein Function | disease:Defects in RSPO1 are the cause of palmoplantar keratoderma with squamous cell carcinoma of skin and sex reversal (PKKSCC) [MIM:610644]. This recessive syndrome is characterized by XX (female to male) SRY-independent sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin.,domain:The FU repeats are required for activation and stabilization of beta-catenin.,function:Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway, possibly via a direct interaction with Wnt proteins, and in a Wnt-independent beta catenin pathway through a receptor signaling pathway that may not use frizzled/LRP receptors. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination.,miscellaneous:Upon injection into mice, it induces rapid onset of crypt cell proliferation involving beta-catenin stabilization. It also displays efficacy in a model of chemotherapy-induced intestinal mucositis suggesting possible therapeutic application in gastrointestinal diseases.,similarity:Belongs to the R-spondin family.,similarity:Contains 1 TSP type-1 domain.,similarity:Contains 2 FU (furin-like) repeats.,subunit:Interacts with the extracelular domain of FZD8 and LRP6. It however does not form a ternary complex with FZD8 and LRP6. Interacts with WNT1. Binds heparin.,tissue specificity:Abundantly expressed in adrenal glands, ovary, testis, thyroid and trachea but not in bonre marrow, spinal cord, stomach, leukocytes colon, small intestine, prostate, thymus and spleen., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |
| Material Safety Data Sheet | |
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