ITM1004
ITM1004
- Catalog: ITM1004
- Gene/Protein: ACTN2/ACTN3
- Product Description: Immunotag™ Actinin-α2 Monoclonal Antibody
504.0000
Price in reward points: 504
| Antibody Specification | |
| Datasheet | 
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| Target Protein | Actinin-α2 |
| Clonality | Monoclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,IF |
| Recommended Dilution | Western Blot: 1/1000 - 1/2000. Immunofluorescence: 1/100 - 1/500. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Rat,Bovine,Chicken,Dog,Pig,Zebrafisheep |
| Host Species | Mouse |
| Immunogen | Purified recombinant human Actinin-α2 (C-terminus) protein fragments expressed in E.coli. |
| Specificity | Actinin-α2 Monoclonal Antibody detects endogenous levels of Actinin-α2 protein. |
| Purification | Affinity purification |
| Form | Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol. |
| Gene Name | ACTN2/ACTN3 |
| Accession No. | P35609 Q9JI91 |
| Alternate Names | ACTN2; Alpha-actinin-2; Alpha-actinin skeletal muscle isoform 2; F-actin cross-linking protein |
| Description | actinin alpha 2(ACTN2) Homo sapiens Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013], |
| Cell Pathway/ Category | Focal adhesion,Adherens_Junction,Adherens_Junction,Leukocyte transendothelial migration,Regulates Actin and Cytoskeleton,Systemic lupus erythematosus,Arrhythmogenic right ventricular cardiomyopathy (ARVC), |
| Protein Expression | Brain,PNS,Skeletal muscle, |
| Subcellular Localization | extracellular region,cytosol,cytoskeleton,actin filament,plasma membrane,focal adhesion,Z disc,filopodium,cortical actin cytoskeleton,platelet alpha granule lumen,pseudopodium,dendritic spine,extracellular exosome, |
| Protein Function | disease:Defects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA) [MIM:612158]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.,function:F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.,similarity:Belongs to the alpha-actinin family.,similarity:Contains 1 actin-binding domain.,similarity:Contains 2 CH (calponin-homology) domains.,similarity:Contains 2 EF-hand domains.,similarity:Contains 4 spectrin repeats.,subcellular location:Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle.,subunit:Homodimer; antiparallel. Also forms heterodimers with ACTN3. Interacts with ADAM12, MYOZ1, MYOZ2 and MYOZ3. Interacts via its C-terminal region with the LDB3 PDZ domain. Interacts with XIRP2.,tissue specificity:Expressed in both skeletal and cardiac muscle., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |
| Material Safety Data Sheet | |
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