ITM1059
ITM1059
- Catalog: ITM1059
- Gene/Protein: KDM1A
- Product Description: Immunotag™ LSD1 Monoclonal Antibody
504.0000
Price in reward points: 504
| Antibody Specification | |
| Datasheet | 
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| Target Protein | LSD1 |
| Clonality | Monoclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,IHC-p,IF |
| Recommended Dilution | Western Blot: 1/1000 - 1/2000. Immunohistochemistry: 1/500 - 1/1000. Immunofluorescence: 1/100 - 1/500. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Mouse,Bovine,Dog,Pig |
| Host Species | Mouse |
| Immunogen | Purified recombinant human LSD1 (N-terminus) protein fragments expressed in E.coli. |
| Specificity | LSD1 Monoclonal Antibody detects endogenous levels of LSD1 protein. |
| Purification | Affinity purification |
| Form | Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol. |
| Gene Name | KDM1A |
| Accession No. | O60341 Q6ZQ88 |
| Alternate Names | KDM1A; AOF2; KDM1; KIAA0601; LSD1; Lysine-specific histone demethylase 1A; BRAF35-HDAC complex protein BHC110; Flavin-containing amine oxidase domain-containing protein 2 |
| Description | lysine demethylase 1A(KDM1A) Homo sapiens This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009], |
| Protein Expression | Brain,Epithelium,Skin,Testis, |
| Subcellular Localization | nuclear chromosome, telomeric region,nuclear chromatin,nucleus,nucleoplasm,transcription factor complex,protein complex,DNA repair complex, |
| Protein Function | cofactor:FAD.,domain:The SWIRN domain may act as an anchor site for a histone tail.,function:Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. May also demethylate 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis.,similarity:Belongs to the flavin monoamine oxidase family.,similarity:Contains 1 SWIRM domain.,subunit:Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, KDM1, RCOR1/CoREST and PHF21A/BHC80. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. In the complex, RCOR1/CoREST strongly enhances the demethylase activity and protects it from the proteasome while PHF21A/BHC80 inhibits the demethylase activity. Interacts with the androgen receptor (AR).,tissue specificity:Ubiquitously expressed., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |
| Material Safety Data Sheet | |
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