ITM1108
ITM1108
- Catalog: ITM1108
- Gene/Protein: TRIM33
- Product Description: Immunotag™ TIF1γ Monoclonal Antibody
504.0000
Price in reward points: 504
| Antibody Specification | |
| Datasheet | 
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| Target Protein | TIF1γ |
| Clonality | Monoclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,IF |
| Recommended Dilution | Western Blot: 1/1000 - 1/2000. Immunofluorescence: 1/100 - 1/500. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Mouse,Rat,Bovine,Dog,Pig |
| Host Species | Mouse |
| Immunogen | Purified recombinant human TIF1γ (C-terminus) protein fragments expressed in E.coli. |
| Specificity | TIF1γ Monoclonal Antibody detects endogenous levels of TIF1γ protein. |
| Purification | Affinity purification |
| Form | Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol. |
| Gene Name | TRIM33 |
| Accession No. | Q9UPN9 Q99PP7 |
| Alternate Names | TRIM33; KIAA1113; RFG7; TIF1G; E3 ubiquitin-protein ligase TRIM33; Ectodermin homolog; RET-fused gene 7 protein; Protein Rfg7; Transcription intermediary factor 1-gamma; TIF1-gamma; Tripartite motif-containing protein 33 |
| Description | tripartite motif containing 33(TRIM33) Homo sapiens The protein encoded by this gene is thought to be a transcriptional corepressor. However, molecules that interact with this protein have not yet been identified. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Three alternatively spliced transcript variants for this gene have been described, however, the full-length nature of one variant has not been determined. [provided by RefSeq, Jul 2008], |
| Protein Expression | Brain,Placenta,Thyroid,Whole embryo, |
| Subcellular Localization | intracellular,nucleus,nucleoplasm,intracellular membrane-bounded organelle, |
| Protein Function | disease:A chromosomal aberration involving TRIM33 is a cause of thyroid papillary carcinoma (PACT) [MIM:188550]. Translocation t(1;10)(p13;q11) with RET. The translocation generates the TRIM33/RET (PTC7) oncogene.,function:Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. According to PubMed:16751102, does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor (By similarity). Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade).,pathway:Protein modification; protein ubiquitination.,similarity:Belongs to the TRIM/RBCC family.,similarity:Contains 1 bromo domain.,similarity:Contains 1 PHD-type zinc finger.,similarity:Contains 1 RING-type zinc finger.,similarity:Contains 2 B box-type zinc fingers.,subcellular location:In discrete nuclear dots resembling nuclear bodies.,subunit:Homooligomer and heterooligomer with TRIM24 and TRIM28 family members. Interacts with SMAD4 in unstimulated cells. Found in a complex with SMAD2 and SMAD3 upon addition of TGF-beta. Interacts with SMAD2 and SMAD3. Interacts with SMAD4 under basal and induced conditions and, upon TGF-beta signaling, with activated SMAD2. Forms a ternary complex with SMAD4 and SMAD2 upon TGF-beta signaling.,tissue specificity:Expressed in stem cells at the bottom of the crypts of the colon (at protein level). Expressed in colon adenomas and adenocarcinomas (at protein level). Expressed in brain, lung, liver, spleen, thymus, prostate, kidney, testis, heart, placenta, pancreas, small intestine, ovary, colon, skeletal muscle and hematopoietic progenitors., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |
| Material Safety Data Sheet | |
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