ITM1323
ITM1323
- Catalog: ITM1323
- Gene/Protein: pkm2
- Product Description: Immunotag™ PKM2 mouse mAb
405.0000
Price in reward points: 405
| Antibody Specification | |
| Datasheet | 
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| Target Protein | PKM2 |
| Clonality | Monoclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,IF |
| Recommended Dilution | wb 1:1000 icc 1:400 |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Mouse,Rat,Monkey |
| Host Species | Mouse |
| Immunogen | Purified recombinant human PKM2 protein fragments expressed in E.coli. |
| Specificity | This antibody detects endogenous levels of PKM2 and does not cross-react with related proteins. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Gene Name | pkm2 |
| Accession No. | P14618 P52480 |
| Alternate Names | CTHBP;Cytosolic thyroid hormone binding protein;Cytosolic thyroid hormone-binding protein;KPYM_HUMAN;MGC3932;OIP 3;OIP-3;OIP3;OPA interacting protein 3;Opa-interacting protein 3;p58;PK muscle type;PK, muscle type;PK2;PK3;PKM;PKM2;pykm;Pyruvate kinase 2/3;Pyruvate kinase 3;Pyruvate kinase isozymes M1/M2;Pyruvate kinase muscle;Pyruvate kinase muscle isozyme;pyruvate kinase PKM;Pyruvate kinase, muscle 2;TCB;THBP1;Thyroid hormone binding protein 1;Thyroid hormone binding protein cytosolic;Thyroid hormone-binding protein 1;Tumor M2 PK;Tumor M2-PK. |
| Description | pyruvate kinase, muscle(PKM) Homo sapiens This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011], |
| Cell Pathway/ Category | Glycolysis / Gluconeogenesis,Purine metabolism,Pyruvate metabolism,Type II diabetes mellitus, |
| Protein Expression | Astrocyte,B-cell lymphoma,Brain,Cajal-Retzius cell,Epithelium,Fetal brain,Kidney,Li |
| Subcellular Localization | nucleus,cytoplasm,mitochondrion,cytosol,plasma membrane,cell-cell adherens junction,cilium,extracellular matrix,vesicle,myelin sheath,extracellular exosome,extracellular vesicle, |
| Protein Function | catalytic activity:ATP + pyruvate = ADP + phosphoenolpyruvate.,cofactor:Divalent metal cations.,cofactor:Magnesium.,cofactor:Potassium.,enzyme regulation:Isoform M2 is allosterically activated by D-fructose 1,6-biphosphate (FBP). Inhibited by oxalate and 3,3',5-triiodo-L-thyronine (T3).,function:Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP.,miscellaneous:There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. L type is major isozyme in the liver, R is found in red cells, M1 is the main form in muscle, heart and brain, and M2 is found in early fetal tissues as well as in most cancer cells.,online information:Pyruvate kinase entry,pathway:Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the pyruvate kinase family.,subunit:Monomer and homotetramer. Exists as a monomer in the absence of FBP, and reversibly associates to form a homotetramer in the presence of FBP. The monomeric form binds T3. Tetramer formation induces pyruvate kinase activity. Interacts with HERC1., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |
| Material Safety Data Sheet | |
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