ITM3453
ITM3453
- Catalog: ITM3453
- Gene/Protein: CDKN1A
- Product Description: Immunotag™ p21 Polyclonal Antibody
385.0000
Price in reward points: 385
| Antibody Specification | |
| Datasheet | 
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| Target Protein | p21 |
| Clonality | Polyclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,IHC-p |
| Recommended Dilution | Western Blot: 1/500 - 1/2000.IHC-p:1:50-300. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Mouse,Rat |
| Host Species | Rabbit |
| Immunogen | Synthesized peptide derived from human p21 around the non-phosphorylation site of T145. |
| Specificity | The antibody detects endogenous p21 protein. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Form | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. |
| Gene Name | CDKN1A |
| Accession No. | P38936 P39689 |
| Alternate Names | CDKN1A; CAP20; CDKN1; CIP1; MDA6; PIC1; SDI1; WAF1; Cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; Melanoma differentiation-associated protein 6; MDA-6; p21 |
| Description | cyclin dependent kinase inhibitor 1A(CDKN1A) Homo sapiens This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lac |
| Cell Pathway/ Category | ErbB_HER,Cell_Cycle_G1S,Cell_Cycle_G2M_DNA,p53,Pathways in cancer,Glioma,Prostate cancer,Melanoma,Bladder cancer,Chronic myeloid leukemia, |
| Protein Expression | Aorta endothelial cell,Epithelium,Eye,Lung, |
| Subcellular Localization | cyclin-dependent protein kinase holoenzyme complex,nucleus,nucleoplasm,nucleolus,cytosol,protein complex,perinuclear region of cytoplasm,PCNA-p21 complex, |
| Protein Function | function:May be the important intermediate by which p53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression.,induction:By p53, mezerein (antileukemic compound) and interferon beta.,PTM:Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA.,similarity:Belongs to the CDI family.,tissue specificity:Expressed in all adult human tissues, with 5-fold lower levels observed in the brain., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |
| Material Safety Data Sheet | |
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