Datasheet |
|
Target Protein |
PKN1 |
Clonality |
Polyclonal |
Storage/Stability |
-20°C/1 year |
Application |
WB,ELISA |
Recommended Dilution |
WB 1:500-2000 ELISA 1:5000-20000 |
Concentration |
1 mg/ml |
Reactive Species |
Human,Mouse,Rat |
Host Species |
Rabbit |
Immunogen |
Synthesized peptide derived from human protein, at AA range: 720-800 |
Specificity |
PKN1 Polyclonal Antibody detects endogenous levels of protein. |
Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
Form |
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide. |
Gene Name |
PKN1 PAK1 PKN PRK1 PRKCL1 |
Accession No. |
Q16512 P70268 Q63433 |
Description |
protein kinase N1(PKN1) Homo sapiens The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008], |
Protein Expression |
Brain,Epithelium,Fetal brain,Hippocampus,Placenta, |
Subcellular Localization |
nucleus,nucleoplasm,cytoplasm,endosome,cytosol,plasma membrane,cytoplasmic, membrane-bounded vesicle,midbody,cleavage furrow,protein complex, |
Protein Function |
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:The C1 domain does not bind the diacylglycerol (DAG).,enzyme regulation:Activated by lipids, particularly cardiolipin and to a lesser extent by other acidic phospholipids. Two specific sites, Thr-774 (activation loop of the kinase domain) and Ser-916 (turn motif), need to be phosphorylated for its full activation.,function:Can phosphorylate ribosomal protein S6. Mediates GTPase Rho dependent intracellular signaling.,PTM:Activated by limited proteolysis with trypsin.,PTM:Autophosphorylated; preferably on serine.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 C2 domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 3 REM (Hr1) repeats.,subunit:Interacts with ZA20D3 (By similarity). Interacts with RhoA and Rac1.,tissue specificity:Found ubiquitously. Expressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas., |
Usage |
For Research Use Only! Not for diagnostic or therapeutic procedures. |