ITN0068
ITN0068
- Catalog: ITN0068
- Gene/Protein: TOPBP1 KIAA0259
- Product Description: Immunotag™ TOPB1 Polyclonal Antibody
385.0000
Price in reward points: 385
| Antibody Specification | |
| Datasheet | 
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| Target Protein | TOPB1 |
| Clonality | Polyclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,ELISA |
| Recommended Dilution | WB 1:500-2000 ELISA 1:5000-20000 |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Mouse |
| Host Species | Rabbit |
| Immunogen | Synthesized peptide derived from human protein . at AA range: 1100-1180 |
| Specificity | TOPB1 Polyclonal Antibody detects endogenous levels of protein. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Form | Liquid in PBS containing 50% glycerol, and 0.02% sodium azide. |
| Gene Name | TOPBP1 KIAA0259 |
| Accession No. | Q92547 Q6ZQF0 |
| Description | topoisomerase (DNA) II binding protein 1(TOPBP1) Homo sapiens This gene encodes a binding protein which interacts with the C-terminal region of topoisomerase II beta. This interaction suggests a supportive role for this protein in the catalytic reactions of topoisomerase II beta through transient breakages of DNA strands. [provided by RefSeq, Jul 2008], |
| Protein Expression | Bone marrow,Brain,Breast,Cervix carcinoma,Clones donated by |
| Subcellular Localization | condensed nuclear chromosome,spindle pole,male germ cell nucleus,nucleus,nucleoplasm,chromosome,cytoplasm,Golgi apparatus,centrosome,actin cytoskeleton,PML body, |
| Protein Function | function:Required for DNA replication. Plays a role in the rescue of stalled replication forks and checkpoint control. Binds double-stranded DNA breaks and nicks as well as single-stranded DNA. Recruits the SWI/SNF chromatin remodeling complex to E2F1-responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage. Induces a large increase in the kinase activity of ATR.,induction:Up-regulated during the S phase of the cell cycle. Up-regulated by E2F1 and interferon.,PTM:Phosphorylated on serine and threonine residues in response to X-ray irradiation.,PTM:Ubiquitinated and degraded by the proteasome. X-ray irradiation reduces ubiquitination.,similarity:Contains 8 BRCT domains.,subcellular location:Detected on unpaired autosomes in meiotic prophase cells. Detected on X and Y chromosomes during later stages of prophase. Colocalizes with ATR and H2AFX at unsynapsed chromosome cores during prophase (By similarity). Has a uniform nuclear distribution during G phase. Colocalizes with BRCA1 at stalled replication forks during S phase. In mitotic cells it colocalizes with BRCA1 at spindle poles and centrosomes during metaphase and anaphase. Detected in discrete foci together with PML and numerous DNA repair enzymes after DNA damage by alkylating agents, UV or gamma irradiation.,subunit:Binds E2F1, PML, RAD9A, SMARCA2, SMARCA4, POLE and UBR5. May interact with TOP2B.,tissue specificity:Highly expressed in heart, brain, placenta, lung and kidney., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |
| Material Safety Data Sheet | |
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