ITP0075
ITP0075
- Catalog: ITP0075
- Gene/Protein: CREB1
- Product Description: Immunotag™ CREB-1 (phospho Ser133) Polyclonal Antibody
490.0000
Price in reward points: 490
| Antibody Specification | |
| Datasheet | 
|
| Target Protein | CREB-1 (Ser133) |
| Clonality | Polyclonal |
| Storage/Stability | -20°C/1 year |
| Application | IF,WB,IHC-p,IP,ELISA |
| Recommended Dilution | IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/10000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Mouse,Rat |
| Host Species | Rabbit |
| Immunogen | Synthesized phospho-peptide around the phosphorylation site of human CREB-1 (phospho Ser133) |
| Specificity | Phospho-CREB-1 (S133) Polyclonal Antibody detects endogenous levels of CREB-1 protein only when phosphorylated at S133. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Gene Name | CREB1 |
| Accession No. | P16220 Q01147 P15337 |
| Alternate Names | CREB1; Cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1 |
| Description | cAMP responsive element binding protein 1(CREB1) Homo sapiens This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016], |
| Cell Pathway/ Category | Antigen processing and presentation,Melanogenesis,Huntington's disease,Prostate cancer, |
| Protein Expression | Eye,Placenta,Spleen,Testis, |
| Subcellular Localization | nuclear chromatin,nucleus,nucleoplasm,nuclear euchromatin,mitochondrial matrix,axon,ATF4-CREB1 transcription factor complex, |
| Protein Function | disease:A chromosomal aberration involving CREB1 is associated with angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type.,function:This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transciption activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.,PTM:Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the bZIP family.,similarity:Contains 1 bZIP domain.,similarity:Contains 1 KID (kinase-inducible) domain.,subunit:Interacts with PPRC1. Binds DNA as a dimer. This dimer is stabilized by magnesium ions. Interacts, through the bZIP domain, with the coactivators TORC1/CRTC1, TORC2/CRTC2 and TORC3/CRTC3. When phosphorylated on Ser-133, binds CREBBP., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |
| Material Safety Data Sheet | |
| English_US |
|
| Danish |
|
| Dutch |
|
| English_UK |
|
| French |
|
| German |
|
| Spanish |
|
| Norwegian |
|
| Portuguese |
|
| Finnish |
|
| Swedish |
|
| Polish |
|