ITP0469
ITP0469
- Catalog: ITP0469
- Gene/Protein: EPS15
- Product Description: Immunotag™ Eps15 (phospho Tyr849) Polyclonal Antibody
490.0000
Price in reward points: 490
| Antibody Specification | |
| Datasheet | 
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| Target Protein | EPS15 (Tyr849) |
| Clonality | Polyclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,ELISA |
| Recommended Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Mouse,Rat |
| Host Species | Rabbit |
| Immunogen | Synthesized phospho-peptide around the phosphorylation site of human Eps15 (phospho Tyr849) |
| Specificity | Phospho-Eps15 (Y849) Polyclonal Antibody detects endogenous levels of Eps15 protein only when phosphorylated at Y849. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Gene Name | EPS15 |
| Accession No. | P42566 P42567 |
| Alternate Names | EPS15; AF1P; Epidermal growth factor receptor substrate 15; Protein Eps15; Protein AF-1p |
| Description | epidermal growth factor receptor pathway substrate 15(EPS15) Homo sapiens This gene encodes a protein that is part of the EGFR pathway. The protein is present at clatherin-coated pits and is involved in receptor-mediated endocytosis of EGF. Notably, this gene is rearranged with the HRX/ALL/MLL gene in acute myelogeneous leukemias. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2009], |
| Cell Pathway/ Category | Endocytosis, |
| Protein Expression | Amygdala,Epithelium,Melanoma,Platelet,Skin,Trachea, |
| Subcellular Localization | cytoplasm,cytosol,plasma membrane,clathrin-coated pit,cell-cell adherens junction,basal plasma membrane,membrane,aggresome,apical plasma membrane,AP-2 adaptor complex,early endosome membrane,intra |
| Protein Function | disease:A chromosomal aberration involving EPS15 is found in acute leukemias. Translocation t(1;11)(p32;q23) with MLL/HRX. The result is a rogue activator protein.,domain:The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.,function:Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits.,PTM:Phosphorylation on Tyr-849 is involved in the internalization of EGFR. Not required for membrane translocation after EGF treatment or for targeting to coated pits, but essential for a subsequent step in EGFR endocytosis (By similarity). Phosphorylated on serine upon DNA damage, probably by ATM or ATR.,similarity:Contains 2 EF-hand domains.,similarity:Contains 2 UIM (ubiquitin-interacting motif) repeats.,similarity:Contains 3 EH domains.,subcellular location:Colocalizes with HGS on bilayered clathrin coats on endosomes.,subcellular location:Recruited to the plasma membrane upon EGFR activation and localizes to coated pits.,subunit:Interacts with HGS; the interaction bridges the interaction of STAM or STAM2 with EPS15. Isoform 2 interacts with HGS and AP2A2. Part of a complex at least composed of EPS15, HGS, and either STAM1 or STAM2. Binds AP2A2 and AP2B1. Binds STON2 and EPN1. Interacts (via its SH3-binding sites) with CRK. Interacts with SH3BP4/TTP.,tissue specificity:Ubiquitously expressed., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |
| Material Safety Data Sheet | |
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