Datasheet |
|
Target Protein |
LIMK-1/2 (Thr508/505) |
Clonality |
Polyclonal |
Storage/Stability |
-20°C/1 year |
Application |
WB,IHC-p,ELISA |
Recommended Dilution |
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
Concentration |
1 mg/ml |
Reactive Species |
Human,Mouse,Rat |
Host Species |
Rabbit |
Immunogen |
Synthesized phospho-peptide around the phosphorylation site of human LIMK-1/2 (phospho Thr508/505) |
Specificity |
Phospho-LIMK-1/2 (T508/505) Polyclonal Antibody detects endogenous levels of LIMK-1/2 protein only when phosphorylated at T508/505. |
Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
Form |
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Gene Name |
LIMK1/LIMK2 |
Accession No. |
P53667/P53671 P53669/P53670 |
Alternate Names |
LIMK1; LIMK; LIM domain kinase 1; LIMK-1; LIMK2; LIM domain kinase 2; LIMK-2 |
Description |
LIM domain kinase 1(LIMK1) Homo sapiens There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cog |
Cell Pathway/ Category |
Axon guidance,Fc gamma R-mediated phagocytosis,Regulates Actin and Cytoskeleton, |
Protein Expression |
Brain,Epidermal carcinoma,Hippocampus,Placenta, |
Subcellular Localization |
nucleoplasm,cytoplasm,cytosol,focal adhesion,membrane,neuron projection, |
Protein Function |
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,disease:Haploinsufficiency of LIMK1 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in Williams-Beuren syndrome (WBS), a rare developmental disorder. It is a contiguous gene deletion syndrome involving genes from chromosome band 7q11.23.,function:Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. May be involved in brain development.,PTM:Autophosphorylated.,PTM:Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.,similarity:Contains 1 PDZ (DHR) domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 LIM zinc-binding domains.,subunit:Self-associates. The LIM domain interacts with the cytoplasmic domain of NRG1. Binds ROCK1. Interacts with SSH1. Interacts with NISCH.,tissue specificity:Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle., |
Usage |
For Research Use Only! Not for diagnostic or therapeutic procedures. |