Datasheet |
|
Target Protein |
IKKβ (Tyr188) |
Clonality |
Polyclonal |
Storage/Stability |
-20°C/1 year |
Application |
WB,IHC-p,ELISA |
Recommended Dilution |
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
Concentration |
1 mg/ml |
Reactive Species |
Human,Mouse,Rat,Monkey |
Host Species |
Rabbit |
Immunogen |
Synthesized phospho-peptide around the phosphorylation site of human IKKβ (phospho Tyr188) |
Specificity |
Phospho-IKKβ (Y188) Polyclonal Antibody detects endogenous levels of IKKβ protein only when phosphorylated at Y188. |
Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
Form |
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Gene Name |
IKBKB |
Accession No. |
O14920 O88351 Q9QY78 |
Alternate Names |
IKBKB; IKKB; Inhibitor of nuclear factor kappa-B kinase subunit beta; I-kappa-B-kinase beta; IKK-B; IKK-beta; IkBKB; I-kappa-B kinase 2; IKK2; Nuclear factor NF-kappa-B inhibitor kinase beta; NFKBIKB |
Description |
inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta(IKBKB) Homo sapiens The protein encoded by this gene phosphorylates the inhibitor in the inhibitor/NF-kappa-B complex, causing dissociation of the inhibitor and activation of NF-kappa-B. The encoded protein itself is found in a complex of proteins. Several transcript variants, some protein-coding and some not, have been found for this gene. [provided by RefSeq, Sep 2011], |
Cell Pathway/ Category |
MAPK_ERK_Growth,MAPK_G_Protein,Chemokine,Apoptosis_Inhibition,Apoptosis_Mitochondrial,Apoptosis_Overview,Toll_Like,NOD-like receptor,RIG-I-like receptor,Cytosolic DNA-sensing pathway,T_Cell_Receptor,B_Cell_Antigen,Neurotrophin,Insulin_Receptor,Adipocytokine,Type II diabetes mellitus,Epithelial cell signaling in Helicobacter pylori infection,Pathways in cancer,Pancreatic cancer,Prostate cancer,Chronic myeloid leukemia,Acute myeloid leukemia,Small cell lung cancer, |
Protein Expression |
Brain,Cervix carcinoma,Hear |
Subcellular Localization |
intracellular,nucleus,cytoplasm,cytosol,IkappaB kinase complex,cytoplasmic side of plasma membrane,CD40 receptor complex,membrane raft, |
Protein Function |
catalytic activity:ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein].,function:Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3.,PTM:Ubiquitination on 'Ser-163' modulates phosphorylation on C-terminal serine residues.,PTM:Upon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response.,PTM:Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, which blocks the I-kappa-B signaling pathway.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Component of the I-kappa-B-kinase (IKK) core complex consisting of CHUK, IKBKB and IKBKG; probably four alpha/CHUK-beta/IKBKB dimers are associated with four gamma/IKBKG subunits. The IKK core complex seems to associate with regulatory or adapter proteins to form a IKK-signalosome holo-complex. Part of a complex composed of NCOA2, NCOA3, CHUK/IKKA, IKBKB, IKBKG and CREBBP. Part of a 70-90 kDa complex at least consisting of CHUK/IKKA, IKBKB, NFKBIA, RELA, IKBKAP and MAP3K14. Interacts with SQSTM1 through PRKCZ or PRKCI. Forms an NGF-induced complex with IKBKB, PRKCI and TRAF6. May interact with MAVS/IPS1. Interacts with NALP2. Interacts with TICAM1. Interacts with Yersinia yopJ. Interacts with FAF1; the interaction disrupts the IKK complex formation. Interacts with ATM. Part of a ternary complex consisting of TANK, IKBKB and IKBKG. Interacts with NIBP; the interaction is direct.,tissue specificity:Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood., |
Usage |
For Research Use Only! Not for diagnostic or therapeutic procedures. |