Datasheet |
|
Target Protein |
Arrestin-β-1 (Ser412) |
Clonality |
Polyclonal |
Storage/Stability |
-20°C/1 year |
Application |
WB,IHC-p,ELISA |
Recommended Dilution |
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
Concentration |
1 mg/ml |
Reactive Species |
Human,Monkey |
Host Species |
Rabbit |
Immunogen |
The antiserum was produced against synthesized peptide derived from human Arrestin 1 around the phosphorylation site of Ser412. AA range:369-418 |
Specificity |
Phospho-Arrestin-β-1 (S412) Polyclonal Antibody detects endogenous levels of Arrestin-β-1 protein only when phosphorylated at S412. |
Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
Form |
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Gene Name |
ARRB1 |
Accession No. |
P49407 Q8BWG8 |
Alternate Names |
ARRB1; ARR1; Beta-arrestin-1; Arrestin beta-1 |
Description |
arrestin beta 1(ARRB1) Homo sapiens Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 1 is a cytosolic protein and acts as a cofactor in the beta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Besides the central nervous system, it is expressed at high levels in peripheral blood leukocytes, and thus the BARK/beta-arrestin system is believed to play a major role in regulating receptor-mediated immune functions. Alternatively spliced transcripts encoding different isoforms of arrestin beta 1 have been described. [provided by RefSeq, Jan 2011], |
Cell Pathway/ Category |
MAPK_ERK_Growth,MAPK_G_Protein,Chemokine,Endocytosis, |
Protein Expression |
Brain,Peripheral blood,Uterus, |
Subcellular Localization |
Golgi membrane,chromatin,nucleus,nucleoplasm,cytoplasm,lysosomal membrane,cytosol,heterotrimeric G-protein complex,plasma membrane,clathrin-coated pit,postsynaptic density,basolateral plasma membrane,cytoplasmic vesicle membr |
Protein Function |
function:Regulates beta-adrenergic receptor function. Beta-arrestins seem to bind phosphorylated beta-adrenergic receptors, thereby causing a significant impairment of their capacity to activate G(S) proteins.,online information:Arrestin entry,similarity:Belongs to the arrestin family., |
Usage |
For Research Use Only! Not for diagnostic or therapeutic procedures. |