ITP0680
ITP0680
- Catalog: ITP0680
- Gene/Protein: WASF1
- Product Description: Immunotag™ WAVE1 (phospho Tyr125) Polyclonal Antibody
490.0000
Price in reward points: 490
| Antibody Specification | |
| Datasheet | 
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| Target Protein | WAVE1 (Tyr125) |
| Clonality | Polyclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,IHC-p,IF,ELISA |
| Recommended Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Mouse,Rat |
| Host Species | Rabbit |
| Immunogen | Synthesized phospho-peptide around the phosphorylation site of human WAVE1 (phospho Tyr125) |
| Specificity | Phospho-WAVE1 (Y125) Polyclonal Antibody detects endogenous levels of WAVE1 protein only when phosphorylated at Y125. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Gene Name | WASF1 |
| Accession No. | Q92558 Q8R5H6 Q5BJU7 |
| Alternate Names | WASF1; KIAA0269; SCAR1; WAVE1; Wiskott-Aldrich syndrome protein family member 1; WASP family protein member 1; Protein WAVE-1; Verprolin homology domain-containing protein 1 |
| Description | WAS protein family member 1(WASF1) Homo sapiens The protein encoded by this gene, a member of the Wiskott-Aldrich syndrome protein (WASP)-family, plays a critical role downstream of Rac, a Rho-family small GTPase, in regulating the actin cytoskeleton required for membrane ruffling. It has been shown to associate with an actin nucleation core Arp2/3 complex while enhancing actin polymerization in vitro. Wiskott-Aldrich syndrome is a disease of the immune system, likely due to defects in regulation of actin cytoskeleton. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008], |
| Cell Pathway/ Category | Adherens_Junction,Fc gamma R-mediated phagocytosis,Regulates Actin and Cytoskeleton, |
| Protein Expression | Bone marrow,Testis, |
| Subcellular Localization | mitochondrial outer membrane,cytoskeleton,focal adhesion,actin cytoskeleton,lamellipodium,SCAR complex,synapse, |
| Protein Function | domain:Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain.,function:Downstream effector molecules involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton.,similarity:Belongs to the SCAR/WAVE family.,similarity:Contains 1 WH2 domain.,subcellular location:Dot-like pattern in the cytoplasm. Concentrated in Rac-regulated membrane-ruffling areas.,subunit:Component of the WAVE1 complex composed of ABI2, CYFIP2, C3orf10/HSPC300, NCKAP1 and WASF1/WAVE1. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1. The complex can also be activated by NCK1 (By similarity). Binds actin and the Arp2/3 complex. Interacts with BAIAP2.,tissue specificity:Highly expressed in brain. Lowly expressed in testis, ovary, colon, kidney, pancreas, thymus, small intestine and peripheral blood., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |
| Material Safety Data Sheet | |
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