Datasheet |
|
Target Protein |
ATP-CS (Ser455) |
Clonality |
Polyclonal |
Storage/Stability |
-20°C/1 year |
Application |
WB,IHC-p,ELISA |
Recommended Dilution |
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
Concentration |
1 mg/ml |
Reactive Species |
Human,Mouse,Rat,Monkey |
Host Species |
Rabbit |
Immunogen |
Synthesized phospho-peptide around the phosphorylation site of human ATP-citrate synthase (phospho Ser455) |
Specificity |
Phospho-ATP-citrate synthase (S455) Polyclonal Antibody detects endogenous levels of ATP-citrate synthase protein only when phosphorylated at S455. |
Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
Form |
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Gene Name |
ACLY |
Accession No. |
P53396 Q91V92 P16638 |
Alternate Names |
ACLY; ATP-citrate synthase; ATP-citrate; pro-S-)-lyase; ACL; Citrate cleavage enzyme |
Description |
ATP citrate lyase(ACLY) Homo sapiens ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2014], |
Cell Pathway/ Category |
Citrate cycle (TCA cycle), |
Protein Expression |
Brain,Epithelium,Hippocampus,Liver,Lymph,Platelet, |
Subcellular Localization |
nucleoplasm,cytoplasm,cytosol,plasma membrane,citrate lyase complex,membrane,extracellular exosome, |
Protein Function |
catalytic activity:ADP + phosphate + acetyl-CoA + oxaloacetate = ATP + citrate + CoA.,function:ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.,similarity:In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.,similarity:In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.,subunit:Homotetramer., |
Usage |
For Research Use Only! Not for diagnostic or therapeutic procedures. |