ITP0715
ITP0715
- Catalog: ITP0715
- Gene/Protein: ALOX5
- Product Description: Immunotag™ 5-LO (phospho Ser272) Polyclonal Antibody
490.0000
Price in reward points: 490
| Antibody Specification | |
| Datasheet | 
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| Target Protein | 5-LO (Ser272) |
| Clonality | Polyclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,IHC-p,ELISA |
| Recommended Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Mouse,Rat |
| Host Species | Rabbit |
| Immunogen | Synthesized phospho-peptide around the phosphorylation site of human 5-LO (phospho Ser272) |
| Specificity | Phospho-5-LO (S272) Polyclonal Antibody detects endogenous levels of 5-LO protein only when phosphorylated at S272. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Gene Name | ALOX5 |
| Accession No. | P09917 P48999 P12527 |
| Alternate Names | ALOX5; LOG5; Arachidonate 5-lipoxygenase; 5-LO; 5-lipoxygenase |
| Description | arachidonate 5-lipoxygenase(ALOX5) Homo sapiens This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012], |
| Cell Pathway/ Category | Arachidonic acid metabolism, |
| Protein Expression | Brain,Spleen, |
| Subcellular Localization | extracellular space,nuclear envelope,nuclear envelope lumen,cytosol,nuclear matrix,nuclear membrane, |
| Protein Function | catalytic activity:Arachidonate + O(2) = leukotriene A(4) + H(2)O.,cofactor:Binds 1 iron ion per subunit.,cofactor:Binds 2 calcium ions per subunit.,function:Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.,pathway:Lipid metabolism; leukotriene A4 biosynthesis.,PTM:Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus.,similarity:Belongs to the lipoxygenase family.,similarity:Contains 1 lipoxygenase domain.,similarity:Contains 1 PLAT domain.,subcellular location:Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association.,subunit:Interacts with ALOX5AP and LTC4S., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |
| Material Safety Data Sheet | |
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