ITP0722
ITP0722
- Catalog: ITP0722
- Gene/Protein: PTPN1
- Product Description: Immunotag™ PTP1B (phospho Ser50) Polyclonal Antibody
490.0000
Price in reward points: 490
| Antibody Specification | |
| Datasheet | 
|
| Target Protein | PTP1B (Ser50) |
| Clonality | Polyclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,IHC-p,ELISA |
| Recommended Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Mouse,Rat,Monkey |
| Host Species | Rabbit |
| Immunogen | Synthesized phospho-peptide around the phosphorylation site of human PTP1B (phospho Ser50) |
| Specificity | Phospho-PTP1B (S50) Polyclonal Antibody detects endogenous levels of PTP1B protein only when phosphorylated at S50. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Gene Name | PTPN1 |
| Accession No. | P18031 P35821 P20417 |
| Alternate Names | PTPN1; PTP1B; Tyrosine-protein phosphatase non-receptor type 1; Protein-tyrosine phosphatase 1B; PTP-1B |
| Description | protein tyrosine phosphatase, non-receptor type 1(PTPN1) Homo sapiens The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of |
| Cell Pathway/ Category | Adherens_Junction,Insulin_Receptor, |
| Protein Expression | Epithelium,Eye,Lymph,Placenta,Tongue,Whole embryo, |
| Subcellular Localization | early endosome,endoplasmic reticulum,cytosol,plasma membrane,cell-cell adherens junction,integral component of membrane,cytoplasmic vesicle,sorting endosome,cytoplasmic side of endoplasmic reticulum membrane, |
| Protein Function | catalytic activity:Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate.,function:May play an important role in CKII- and p60c-src-induced signal transduction cascades.,PTM:Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.,similarity:Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.,similarity:Contains 1 tyrosine-protein phosphatase domain., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |
| Material Safety Data Sheet | |
| English_US |
|
| Danish |
|
| Dutch |
|
| English_UK |
|
| French |
|
| German |
|
| Spanish |
|
| Norwegian |
|
| Portuguese |
|
| Finnish |
|
| Swedish |
|
| Polish |
|