ITP0897
ITP0897
- Catalog: ITP0897
- Gene/Protein: TNK2
- Product Description: Immunotag™ ACK (phospho Tyr284) Polyclonal Antibody
490.0000
Price in reward points: 490
| Antibody Specification | |
| Datasheet | 
|
| Target Protein | ACK (Tyr284) |
| Clonality | Polyclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,IHC-p,IF,ELISA |
| Recommended Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Mouse |
| Host Species | Rabbit |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ACK1 around the phosphorylation site of Tyr284. AA range:250-299 |
| Specificity | Phospho-ACK (Y284) Polyclonal Antibody detects endogenous levels of ACK protein only when phosphorylated at Y284. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Gene Name | TNK2 |
| Accession No. | Q07912 O54967 |
| Alternate Names | TNK2; ACK1; Activated CDC42 kinase 1; ACK-1; Tyrosine kinase non-receptor protein 2 |
| Description | tyrosine kinase non receptor 2(TNK2) Homo sapiens This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq, Jul 2008], |
| Protein Expression | Brain,Epithelium,Hippocampus,Thalamus,Uterus, |
| Subcellular Localization | nucleus,cytoplasm,endosome,plasma membrane,clathrin-coated pit,adherens junction,membrane,clathrin-coated vesicle,cytoplasmic vesicle membrane,extrinsic component of cytoplasmic side of plasma membrane,Grb2-EGFR complex, |
| Protein Function | catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,cofactor:Magnesium.,enzyme regulation:The SH3 domain appears to play an autoinhibitory role.,function:Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR. Participates in clathrin-mediated endocytosis. May be involved both in adult synaptic function and plasticity and in brain development.,sequence caution:Unlikely isoform. Aberrant splice sites.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Contains 1 CRIB domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH3 domain.,subunit:Interacts with CDC42. Interacts with activated CSPG4., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |
| Material Safety Data Sheet | |
| English_US |
|
| Danish |
|
| Dutch |
|
| English_UK |
|
| French |
|
| German |
|
| Spanish |
|
| Norwegian |
|
| Portuguese |
|
| Finnish |
|
| Swedish |
|
| Polish |
|