ITP1044
ITP1044
- Catalog: ITP1044
- Gene/Protein: ILK
- Product Description: Immunotag™ ILK (phospho Ser246) Polyclonal Antibody
490.0000
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Antibody Specification | |
Datasheet | |
Target Protein | IL-K (Ser246) |
Clonality | Polyclonal |
Storage/Stability | -20°C/1 year |
Application | IHC-p,ELISA |
Recommended Dilution | Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
Concentration | 1 mg/ml |
Reactive Species | Human,Mouse,Rat |
Host Species | Rabbit |
Immunogen | Synthesized phospho-peptide around the phosphorylation site of human ILK (phospho Ser246) |
Specificity | Phospho-ILK (S246) Polyclonal Antibody detects endogenous levels of ILK protein only when phosphorylated at S246. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Gene Name | ILK |
Accession No. | Q13418 O55222 Q99J82 |
Alternate Names | ILK; ILK1; ILK2; Integrin-linked protein kinase; 59 kDa serine/threonine-protein kinase; ILK-1; ILK-2; p59ILK |
Description | integrin linked kinase(ILK) Homo sapiens This gene encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates integrin-mediated signal transduction. Activity of this protein is important in the epithelial to mesenchymal transition, and over-expression of this gene is implicated in tumor growth and metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013], |
Cell Pathway/ Category | PPAR,Focal adhesion,Endometrial cancer, |
Protein Expression | Brain,Cervix,Endometrium,Placenta, |
Subcellular Localization | stress fiber,nucleoplasm,cytoplasm,cytosol,plasma membrane,cell-cell junction,focal adhesion,membrane,sarcomere,lamellipodium,cell junction,extracellular matrix,neuronal cell body,costamere,termi |
Protein Function | catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:A PH-like domain is involved in phosphatidylinositol phosphate binding.,enzyme regulation:Stimulated rapidly but transiently by both cell fibronectin interactions, as well as by insulin, in a PI3-K-dependent manner, likely via the binding of PtdIns(3,4,5)P3 with a PH-like domain of ILK.,function:Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.,PTM:Autophosphorylated on serine residues.,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.,similarity:Contains 1 protein kinase domain.,similarity:Contains 5 ANK repeats.,subunit:Interacts with cytoplasmic domain of beta 1 subunit of integrin. Could also interacts with beta 2, beta 3 and/or beta 5 subunit of integrin. Interacts (via ANK repeats) with LIMS1 and LIMS2. Interacts with parvins and probably TGFB1I1.,tissue specificity:Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver., |
Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |