ITP1072
ITP1072
- Catalog: ITP1072
- Gene/Protein: ITCH
- Product Description: Immunotag™ AIP4 (phospho Tyr420) Polyclonal Antibody
490.0000
Price in reward points: 490
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Antibody Specification | |
Datasheet | |
Target Protein | AIP4 (Tyr420) |
Clonality | Polyclonal |
Storage/Stability | -20°C/1 year |
Application | IHC-p,ELISA |
Recommended Dilution | Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
Concentration | 1 mg/ml |
Reactive Species | Human,Mouse |
Host Species | Rabbit |
Immunogen | The antiserum was produced against synthesized peptide derived from human ITCH around the phosphorylation site of Tyr420. AA range:386-435 |
Specificity | Phospho-AIP4 (Y420) Polyclonal Antibody detects endogenous levels of AIP4 protein only when phosphorylated at Y420. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Gene Name | ITCH |
Accession No. | Q96J02 Q8C863 |
Alternate Names | ITCH; E3 ubiquitin-protein ligase Itchy homolog; Itch; Atrophin-1-interacting protein 4; AIP4; NFE2-associated polypeptide 1; NAPP1 |
Description | itchy E3 ubiquitin protein ligase(ITCH) Homo sapiens This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012], |
Cell Pathway/ Category | Ubiquitin mediated proteolysis,Endocytosis, |
Protein Expression | Aorta endothelial cell,B-cell,Fetal brain,Kidney,Leukemia,Placenta,Trachea, |
Subcellular Localization | nucleus,cytoplasm,cytosol,plasma membrane,membrane,extracellular exosome, |
Protein Function | function:E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Regulates the transcriptional activity of several transcription factors, and probably plays an important role in the regulation of immune response.,pathway:Protein modification; protein ubiquitination.,PTM:Phosphorylated on tyrosine residues.,similarity:Contains 1 C2 domain.,similarity:Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.,similarity:Contains 4 WW domains.,subunit:Interacts via its WW domains with DRPLA, NFE2 and CBLC. Interacts with Epstein-Barr virus LMP2A. Interacts with NOTCH1, OCLN, JUN and JUNB. Interacts with NDFIP1 in vitro (By similarity). Interacts with ARHGEF7.,tissue specificity:Widely expressed., |
Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |