Datasheet |
|
Target Protein |
MAP-4 (Ser696) |
Clonality |
Polyclonal |
Storage/Stability |
-20°C/1 year |
Application |
IHC-p,IF,ELISA |
Recommended Dilution |
Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
Concentration |
1 mg/ml |
Reactive Species |
Human,Mouse,Rat |
Host Species |
Rabbit |
Immunogen |
Synthesized phospho-peptide around the phosphorylation site of human MAP-4 (phospho Ser696) |
Specificity |
Phospho-MAP-4 (S696) Polyclonal Antibody detects endogenous levels of MAP-4 protein only when phosphorylated at S696. |
Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
Form |
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Gene Name |
MAP4 |
Accession No. |
P27816 P27546 Q5M7W5 |
Alternate Names |
MAP4; Microtubule-associated protein 4; MAP-4 |
Description |
microtubule associated protein 4(MAP4) Homo sapiens The protein encoded by this gene is a major non-neuronal microtubule-associated protein. This protein contains a domain similar to the microtubule-binding domains of neuronal microtubule-associated protein (MAP2) and microtubule-associated protein tau (MAPT/TAU). This protein promotes microtubule assembly, and has been shown to counteract destabilization of interphase microtubule catastrophe promotion. Cyclin B was found to interact with this protein, which targets cell division cycle 2 (CDC2) kinase to microtubules. The phosphorylation of this protein affects microtubule properties and cell cycle progression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008], |
Protein Expression |
Bone marrow,Brain,Cerebellum,Colon carcinoma,Epithelium,Eye,Heart,Liver,Lym |
Subcellular Localization |
cytoplasm,microtubule,microtubule associated complex,plasma membrane,axoneme,postsynaptic density,microtubule cytoskeleton,neuron projection,extracellular exosome,mitotic spindle, |
Protein Function |
Additional isoforms seem to exist,caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,function:Non-neuronal microtubule-associated protein. Promotes microtubule assembly.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR (By similarity). Phosphorylation on Ser-787 negatively regulates MAP4 activity to promote microtubule assembly. Isoform 3 is phosphorylated on Ser-337 and Ser-338.,similarity:Contains 3 Tau/MAP repeats.,similarity:Contains 4 Tau/MAP repeats., |
Usage |
For Research Use Only! Not for diagnostic or therapeutic procedures. |