ITT0118
ITT0118
- Catalog: ITT0118
- Gene/Protein: ADAR
- Product Description: Immunotag™ ADAR1 Polyclonal Antibody
385.0000
Price in reward points: 385
| Antibody Specification | |
| Datasheet | 
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| Target Protein | ADAR1 |
| Clonality | Polyclonal |
| Storage/Stability | -20°C/1 year |
| Application | IHC-p,WB,ELISA |
| Recommended Dilution | WB 1:500-2000 Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Mouse,Rat |
| Host Species | Rabbit |
| Immunogen | Synthesized peptide derived from ADAR1, at AA range: 1140-1220 |
| Specificity | ADAR1 Polyclonal Antibody detects endogenous levels of ADAR1 protein. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Gene Name | ADAR |
| Accession No. | P55265 Q99MU3 P55266 |
| Alternate Names | ADAR; ADAR1; DSRAD; G1P1; IFI4; Double-stranded RNA-specific adenosine deaminase; DRADA; 136 kDa double-stranded RNA-binding protein; p136; Interferon-inducible protein 4; IFI-4; K88DSRBP |
| Description | adenosine deaminase, RNA specific(ADAR) Homo sapiens This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010], |
| Cell Pathway/ Category | Cytosolic DNA-sensing pathway, |
| Protein Expression | Amygdala,Aorta endothelial cell,Cervix carcinoma,Epithelium,Fetal kidney,Ki |
| Subcellular Localization | nucleus,nucleoplasm,nucleolus,cytoplasm,membrane,supraspliceosomal complex, |
| Protein Function | caution:The N-terminus of isoform 4 has been derived from EST and genomic sequences.,disease:Defects in ADAR are a cause of dyschromatosis symmetrical hereditaria (DSH) [MIM:127400]; also known as reticulate acropigmentation of Dohi. DSH is a pigmentary genodermatosis of autosomal dominant inheritance characterized by a mixture of hyperpigmented and hypopigmented macules distributed on the dorsal parts of the hands and feet.,function:Converts multiple adenosines to inosines and creates I/U mismatched base pairs in double-helical RNA substrates without apparent sequence specificity. Has been found to modify more frequently adenosines in AU-rich regions, probably due to the relative ease of melting A/U base pairs as compared to G/C pairs. Functions to modify viral RNA genomes and may be responsible for hypermutation of certain negative-stranded viruses. Edits the messenger RNAs for glutamate receptor (GLUR) subunits by site-selective adenosine deamination. Produces low-level editing at the GLUR-B Q/R site, but edits efficiently at the R/G site and HOTSPOT1. Binds to short interfering RNAs (siRNA) without editing them and suppresses siRNA-mediated RNA interference. Binds to ILF3/NF90 and up-regulates ILF3-mediated gene expression.,induction:Isoform 1 is induced by interferon alpha. Isoform 5 is constitutively expressed.,PTM:Sumoylation reduces RNA-editing activity.,similarity:Contains 1 A to I editase domain.,similarity:Contains 2 DRADA repeats.,similarity:Contains 3 DRBM (double-stranded RNA-binding) domains.,subcellular location:Isoform 1 is found predominantly in cytoplasm but appears to shuttle between the cytoplasm and nucleus. Isoform 5 is found exclusively in the nucleolus.,subunit:Homodimer. Isoform 1 interacts with ILF2/NF45 and ILF3/NF90.,tissue specificity:Ubiquitously expressed, highest levels were found in brain and lung., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |
| Material Safety Data Sheet | |
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