ITT0306
ITT0306
- Catalog: ITT0306
- Gene/Protein: ARFGAP1
- Product Description: Immunotag™ ARF GAP1 Polyclonal Antibody
385.0000
Price in reward points: 385
| Antibody Specification | |
| Datasheet | 
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| Target Protein | ARF GAP1 |
| Clonality | Polyclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,IHC-p,IF,ELISA |
| Recommended Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Mouse,Rat,Monkey |
| Host Species | Rabbit |
| Immunogen | Synthesized peptide derived from ARF GAP1, at AA range: 140-220 |
| Specificity | ARF GAP1 Polyclonal Antibody detects endogenous levels of ARF GAP1 protein. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Gene Name | ARFGAP1 |
| Accession No. | Q8N6T3 Q9EPJ9 Q62848 |
| Alternate Names | ARFGAP1; ARF1GAP; ADP-ribosylation factor GTPase-activating protein 1; ARF GAP 1; ADP-ribosylation factor 1 GTPase-activating protein; ARF1 GAP; ARF1-directed GTPase-activating protein |
| Description | ADP ribosylation factor GTPase activating protein 1(ARFGAP1) Homo sapiens The protein encoded by this gene is a GTPase-activating protein, which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013], |
| Cell Pathway/ Category | Endocytosis, |
| Protein Expression | Brain,Epithelium,Fetal brain,Testis, |
| Subcellular Localization | Golgi apparatus,cytosol,postsynaptic density,synapse, |
| Protein Function | domain:The region downstream of Arf-GAP domain is essential to GAP activity in vivo. This region may be required for its targeting to Golgi membranes.,function:GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and RNP24. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine.,sequence caution:Intron retention.,similarity:Contains 1 Arf-GAP domain.,subcellular location:Associates with the Golgi complex.,subunit:Interacts with ARF1. Interacts with the COPI coat proteins, KDELR1 and RNP24. The interaction with RNP24 inhibits the GAP activity., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |
| Material Safety Data Sheet | |
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