ITT0329
ITT0329
- Catalog: ITT0329
- Gene/Protein: RNF111
- Product Description: Immunotag™ Arkadia Polyclonal Antibody
385.0000
Price in reward points: 385
| Antibody Specification | |
| Datasheet | 
|
| Target Protein | Arkadia |
| Clonality | Polyclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,ELISA |
| Recommended Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Mouse |
| Host Species | Rabbit |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RNF111. AA range:901-950 |
| Specificity | Arkadia Polyclonal Antibody detects endogenous levels of Arkadia protein. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Gene Name | RNF111 |
| Accession No. | Q6ZNA4 Q99ML9 |
| Alternate Names | RNF111; E3 ubiquitin-protein ligase Arkadia; RING finger protein 111 |
| Description | ring finger protein 111(RNF111) Homo sapiens The protein encoded by this gene is a nuclear RING-domain containing E3 ubiquitin ligase. This protein interacts with the transforming growth factor (TGF) -beta/NODAL signaling pathway by promoting the ubiquitination and proteosomal degradation of negative regulators, like SMAD proteins, and thereby enhances TGF-beta target-gene transcription. As a modulator of the nodal signaling cascade, this gene plays a critical role in the induction of mesoderm during embryonic development. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012], |
| Protein Expression | Amygdala,Brain,Colon,Endometrium,Placenta,Thymus,Tongue, |
| Subcellular Localization | nucleus,nucleoplasm,nucleolus,cytoplasm,protein complex, |
| Protein Function | function:Acts in the NODAL pathway of mesoderm patterning during embryonic development. Acts downstream AXIN1 as an E3 ubiquitin-protein ligase which promotes the ubiquitination of inhibitory SMADs such as SMAD7, induces their proteasomal degradation and thereby enhances the transcriptional activity of TGF-beta and BMP. Activates Smad3/Smad4-dependent transcription by triggering signal-induced SnoN degradation.,pathway:Protein modification; protein ubiquitination.,similarity:Contains 1 RING-type zinc finger.,subcellular location:Upon TGF-beta treatment, translocates from nucleus to cytosol.,subunit:Interacts with SMAD6, SMAD7, AXIN1, AXIN2 and SKIL isoform SNON. Part of a complex containing RNF111, AXIN1 and SMAD7.,tissue specificity:Broadly expressed., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |
| Material Safety Data Sheet | |
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