ITT0593
ITT0593
- Catalog: ITT0593
- Gene/Protein: CAD
- Product Description: Immunotag™ CAD Polyclonal Antibody
385.0000
Price in reward points: 385
| Antibody Specification | |
| Datasheet | 
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| Target Protein | CAD |
| Clonality | Polyclonal |
| Storage/Stability | -20°C/1 year |
| Application | IHC-p,ELISA |
| Recommended Dilution | Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Mouse |
| Host Species | Rabbit |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CAD. AA range:422-471 |
| Specificity | CAD Polyclonal Antibody detects endogenous levels of CAD protein. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Gene Name | CAD |
| Accession No. | P27708 |
| Alternate Names | CAD; CAD protein |
| Description | carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase(CAD) Homo sapiens The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015], |
| Cell Pathway/ Category | Pyrimidine metabolism,Alanine, aspartate and glutamate metabolism, |
| Protein Expression | Colon adenocarcinoma,Epithe |
| Subcellular Localization | nucleus,nucleoplasm,cytosol,membrane,nuclear matrix,cell projection,neuronal cell body,terminal bouton,protein complex,extracellular exosome, |
| Protein Function | catalytic activity:(S)-dihydroorotate + H(2)O = N-carbamoyl-L-aspartate.,catalytic activity:2 ATP + L-glutamine + HCO(3)(-) + H(2)O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.,catalytic activity:Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate.,cofactor:Binds 1 zinc ion per subunit (for dihydroorotase activity) .,enzyme regulation:Allosterically regulated and controlled by phosphorylation. 5-phosphoribose 1-diphosphate is an activator while UMP is an inhibitor of the CPSase reaction.,function:This protein is a "fusion" protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase).,miscellaneous:GATase (glutamine amidotransferase) and CPSase (carbamoyl phosphate synthase) form together the glutamine-dependent CPSase (GD-CPSase) (EC 6.3.5.5).,online information:Aspartate carbamoyltransferase entry,pathway:Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 1/6.,pathway:Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 2/6.,pathway:Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 3/6.,similarity:Belongs to the ATCase/OTCase family.,similarity:Contains 1 glutamine amidotransferase type-1 domain.,similarity:Contains 2 ATP-grasp domains.,similarity:In the central section; belongs to the DHOase family.,subunit:Homohexamer., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |
| Material Safety Data Sheet | |
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