ITT0791
ITT0791
- Catalog: ITT0791
- Gene/Protein: CDC20
- Product Description: Immunotag™ Cdc20 Polyclonal Antibody
385.0000
Price in reward points: 385
| Antibody Specification | |
| Datasheet | 
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| Target Protein | CDC20 |
| Clonality | Polyclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,IHC-p,ELISA |
| Recommended Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Mouse,Rat |
| Host Species | Rabbit |
| Immunogen | Synthesized peptide derived from Cdc20, at AA range: 50-130 |
| Specificity | Cdc20 Polyclonal Antibody detects endogenous levels of Cdc20 protein. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Gene Name | CDC20 |
| Accession No. | Q12834 Q9JJ66 Q62623 |
| Alternate Names | CDC20; Cell division cycle protein 20 homolog; p55CDC |
| Description | cell division cycle 20(CDC20) Homo sapiens CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation. [provided by RefSeq, Jul 2008], |
| Cell Pathway/ Category | Cell_Cycle_G1S,Cell_Cycle_G2M_DNA,Oocyte meiosis,Ubiquitin mediated proteolysis, |
| Protein Expression | Colon,Colon adenocarcinoma,Liver,Lymph,Muscle,Ovary,Skin,Spleen,Testis, |
| Subcellular Localization | spindle pole,nucleus,nucleoplasm,anaphase-promoting complex,cytoplasm,centrosome,spindle,cytosol,protein complex,perinuclear region of cytoplasm, |
| Protein Function | developmental stage:Synthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition.,function:Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates.,pathway:Protein modification; protein ubiquitination.,PTM:Phosphorylated during mitosis, probably by maturation promoting factor (MPF).,PTM:Ubiquitinated and degraded by the proteasome during spindle assembly checkpoint.,similarity:Belongs to the WD repeat CDC20/Fizzy family.,similarity:Contains 7 WD repeats.,subunit:Found in a complex with CDC20, CDC27, SPATC1 and TUBG1. Interacts with SPATC1 (By similarity). Interacts with MAD2L1 and BUB1B. The phosphorylated form interacts with APC/C., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |
| Material Safety Data Sheet | |
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