ITT1364
ITT1364
- Catalog: ITT1364
- Gene/Protein: LIG1
- Product Description: Immunotag™ DNA Ligase I Polyclonal Antibody
385.0000
Price in reward points: 385
| Antibody Specification | |
| Datasheet | 
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| Target Protein | DNA Ligase I |
| Clonality | Polyclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,IHC-p,ELISA |
| Recommended Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human |
| Host Species | Rabbit |
| Immunogen | The antiserum was produced against synthesized peptide derived from human DNL1. AA range:111-160 |
| Specificity | DNA Ligase I Polyclonal Antibody detects endogenous levels of DNA Ligase I protein. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Gene Name | LIG1 |
| Accession No. | P18858 P37913 |
| Alternate Names | LIG1; DNA ligase 1; DNA ligase I; Polydeoxyribonucleotide synthase [ATP] 1 |
| Description | DNA ligase 1(LIG1) Homo sapiens This gene encodes a member of the ATP-dependent DNA ligase protein family. The encoded protein functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of this gene may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014], |
| Cell Pathway/ Category | DNA replication,Base excision repair,Nucleotide excision repair,Mismatch repair, |
| Protein Expression | Brain,Epithelium,Eye,PCR rescued clones,T lymphoblast, |
| Subcellular Localization | nucleus,nucleoplasm,mitochondrion,Golgi apparatus,intracellular membrane-bounded organelle, |
| Protein Function | catalytic activity:ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).,cofactor:Magnesium.,disease:Defects in LIG1 seem to cause immunodeficiencies and cellular hypersensitivity to DNA-damaging agents.,function:DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.,online information:DNA ligase entry,online information:LIG1 mutation db,similarity:Belongs to the ATP-dependent DNA ligase family., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |
| Material Safety Data Sheet | |
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