ITT1391
ITT1391
- Catalog: ITT1391
- Gene/Protein: DOCK1
- Product Description: Immunotag™ DOCK 180 Polyclonal Antibody
385.0000
Price in reward points: 385
| Antibody Specification | |
| Datasheet | 
|
| Target Protein | DOCK 180 |
| Clonality | Polyclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,ELISA |
| Recommended Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Mouse |
| Host Species | Rabbit |
| Immunogen | The antiserum was produced against synthesized peptide derived from human DOCK1. AA range:1661-1710 |
| Specificity | DOCK 180 Polyclonal Antibody detects endogenous levels of DOCK 180 protein. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Gene Name | DOCK1 |
| Accession No. | Q14185 Q8BUR4 |
| Alternate Names | DOCK1; Dedicator of cytokinesis protein 1; 180 kDa protein downstream of CRK; DOCK180 |
| Description | dedicator of cytokinesis 1(DOCK1) Homo sapiens This gene encodes a member of the dedicator of cytokinesis protein family. Dedicator of cytokinesis proteins act as guanine nucleotide exchange factors for small Rho family G proteins. The encoded protein regulates the small GTPase Rac, thereby influencing several biological processes, including phagocytosis and cell migration. Overexpression of this gene has also been associated with certain cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014], |
| Cell Pathway/ Category | Focal adhesion,Regulates Actin and Cytoskeleton, |
| Protein Expression | Epithelium,Placenta, |
| Subcellular Localization | intracellular,nucleus,nucleoplasm,cytoplasm,cytosol,membrane,guanyl-nucleotide exchange factor complex, |
| Protein Function | domain:The DHR-2 domain is necessary and sufficient for the GEF activity.,function:Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1.,similarity:Belongs to the DOCK family.,similarity:Contains 1 DHR-1 (CZH-1) domain.,similarity:Contains 1 DHR-2 (CZH-2) domain.,similarity:Contains 1 SH3 domain.,subcellular location:Recruited to membranes via its interaction with phosphatidylinositol 3,4,5-triphosphate.,subunit:Interacts with the SH3 domains of CRK and NCK2 via multiple sites. Interacts with nucleotide-free RAC1 via its DHR-2 domain. Interacts with ELMO1, ELMO2 and probably ELMO3 via its SH3 domain. Interacts with RAC1 and BAI1.,tissue specificity:Highly expressed in placenta, lung, kidney, pancreas and ovary. Expressed at intermediate level in thymus, testes and colon., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |
| Material Safety Data Sheet | |
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