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  • Gene/Protein: EXO1
  • Product Description: Immunotag™ Exo1 Polyclonal Antibody
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Immunotag™ Exo1 Polyclonal Antibody
Antibody Specification
Target Protein Exo1
Clonality Polyclonal
Storage/Stability -20°C/1 year
Application WB,IF,ELISA
Recommended Dilution Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Concentration 1 mg/ml
Reactive Species Human,Mouse
Host Species Rabbit
Immunogen The antiserum was produced against synthesized peptide derived from human EXO1. AA range:61-110
Specificity Exo1 Polyclonal Antibody detects endogenous levels of Exo1 protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Form Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene Name EXO1
Accession No. Q9UQ84 Q9QZ11
Alternate Names EXO1; EXOI; HEX1; Exonuclease 1; hExo1; Exonuclease I; hExoI
Description exonuclease 1(EXO1) Homo sapiens This gene encodes a protein with 5' to 3' exonuclease activity as well as an RNase H activity. It is similar to the Saccharomyces cerevisiae protein Exo1 which interacts with Msh2 and which is involved in mismatch repair and recombination. Alternative splicing of this gene results in three transcript variants encoding two different isoforms. [provided by RefSeq, Jul 2008],
Cell Pathway/ Category Mismatch repair,
Protein Expression Epithelium,Skin,Sperm,Testis,
Subcellular Localization nucleus,nucleoplasm,cytoplasm,
Protein Function cofactor:Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.,developmental stage:Highly expressed in fetal liver and at lower levels in fetal brain, heart, kidney, spleen and thymus.,function:5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis.,polymorphism:Most naturally occurring variants in this protein are not associated with familial disposition to hereditary non-polyposis colorectal cancer (HNPCC). Furthermore, germline deletions involving this locus are not associated with clinically manifested colorectal tumors.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily.,subcellular location:Colocalizes with PCNA to discrete nuclear foci in S-phase.,subunit:Interacts with the MLH1-PMS2 heterodimer via MLH1. Interacts with MSH3. Interacts with the MSH2-MSH6 heterodimer via MSH2, and this interaction may increase the processivity of the 5'->3' exonuclease activity. Interacts with PCNA, and this interaction may both stimulate the cryptic 3'->5' exonuclease activity and suppress the 5'->3' exonuclease activity. Interacts with WRN, and this interaction stimulates both the 5'->3' exonuclease activity and cleavage of 5'-overhanging flap structures. Interacts with RECQL/RECQ1, and this interaction stimulates cleavage of 5'-overhanging flap structures.,tissue specificity:Highly expressed in bone marrow, testis and thymus. Expressed at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach.,
Usage For Research Use Only! Not for diagnostic or therapeutic procedures.
Material Safety Data Sheet

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