ITT2227
ITT2227
- Catalog: ITT2227
- Gene/Protein: EIF2AK1
- Product Description: Immunotag™ HRI Polyclonal Antibody
385.0000
Price in reward points: 385
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Antibody Specification | |
Datasheet | |
Target Protein | HRI |
Clonality | Polyclonal |
Storage/Stability | -20°C/1 year |
Application | WB,IHC-p,ELISA |
Recommended Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
Concentration | 1 mg/ml |
Reactive Species | Human,Mouse,Monkey |
Host Species | Rabbit |
Immunogen | The antiserum was produced against synthesized peptide derived from human EIF2AK1. AA range:571-620 |
Specificity | HRI Polyclonal Antibody detects endogenous levels of HRI protein. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Gene Name | EIF2AK1 |
Accession No. | Q9BQI3 Q9Z2R9 |
Alternate Names | EIF2AK1; HRI; KIAA1369; Eukaryotic translation initiation factor 2-alpha kinase 1; Heme-controlled repressor; HCR; Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase; Heme-regulated inhibitor; Hemin-sensitive initiation factor 2 |
Description | eukaryotic translation initiation factor 2 alpha kinase 1(EIF2AK1) Homo sapiens The protein encoded by this gene acts at the level of translation initiation to downregulate protein synthesis in response to stress. The encoded protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008], |
Protein Expression | Amygdala,Bone marrow,Brain,Epithelium,Fetal |
Subcellular Localization | cytoplasm, |
Protein Function | catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Hemin inactivates EIF2AK1 by promoting the formation of a disulfide-linked homodimer. Binding of nitric oxide (NO) to the heme iron in the N-terminal heme-binding domain activates the kinase activity, while binding of carbon monoxide (CO) suppresses kinase activity.,function:Mediates down-regulation of protein synthesis in response to various stress conditions by the phosphorylation of EIF2S1 at 'Ser-48' and 'Ser-51'. Protein synthesis is inhibited at the level of initiation.,miscellaneous:Can bind 2 molecules of heme per polypeptide chain.,PTM:Activated by autophosphorylation; phosphorylated predominantly on serine and threonine residues, but also on tyrosine residues.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 HRM (heme regulatory motif) repeats.,subunit:Synthesized in an inactive form that binds to the N-terminal domain of CDC37. Has to be associated with a multiprotein complex containing Hsp90, CDC37 and PPP5C for maturation and activation by autophosphorylation. The phosphatase PPP5C modulates this activation. Homodimer; non-covalently bound in the absence of hemin. Converted to an inactive disulfide linked homodimer in the presence of hemin.,tissue specificity:Detected in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, kidney, spleen, muscle and stomach., |
Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |