ITT3595
ITT3595
- Catalog: ITT3595
- Gene/Protein: PARP3
- Product Description: Immunotag™ PARP-3 Polyclonal Antibody
385.0000
Price in reward points: 385
| Antibody Specification | |
| Datasheet | 
|
| Target Protein | PARP-3 |
| Clonality | Polyclonal |
| Storage/Stability | -20°C/1 year |
| Application | IHC-p,ELISA |
| Recommended Dilution | Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human |
| Host Species | Rabbit |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PARP3. AA range:10-59 |
| Specificity | PARP-3 Polyclonal Antibody detects endogenous levels of PARP-3 protein. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Gene Name | PARP3 |
| Accession No. | Q9Y6F1 |
| Alternate Names | PARP3; ADPRT3; ADPRTL3; Poly [ADP-ribose] polymerase 3; PARP-3; hPARP-3; ADP-ribosyltransferase diphtheria toxin-like 3; ARTD3; IRT1; NAD(+) ADP-ribosyltransferase 3; ADPRT-3; Poly[ADP-ribose] synthase 3; pADPRT-3 |
| Description | poly(ADP-ribose) polymerase family member 3(PARP3) Homo sapiens The protein encoded by this gene belongs to the PARP family. These enzymes modify nuclear proteins by poly-ADP-ribosylation, which is required for DNA repair, regulation of apoptosis, and maintenance of genomic stability. This gene encodes the poly(ADP-ribosyl)transferase 3, which is preferentially localized to the daughter centriole throughout the cell cycle. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008], |
| Cell Pathway/ Category | Base excision repair, |
| Protein Expression | B-cell,Brain,Fetal brain,Frontal cortex,Kidney, |
| Subcellular Localization | nucleus,cytoplasm,centriole,site of double-strand break, |
| Protein Function | catalytic activity:NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.,domain:According to PubMed:10329013 the N-terminal domain (54 amino acids) of isoform 2 is responsible for its centrosomal localization. The C-terminal region contains the catalytic domain.,function:Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. May link the DNA damage surveillance network to the mitotic fidelity checkpoint. Negatively influences the G1/S cell cycle progression without interfering with centrosome duplication. Binds DNA. May be involved in the regulation of PRC2 and PRC3 complex-dependent gene silencing.,PTM:Auto-poly(ADP)-ribosylation.,similarity:Contains 1 PARP alpha-helical domain.,similarity:Contains 1 PARP catalytic domain.,subcellular location:Core component of the centrosome. Preferentially localized to the daughter centriole throughout the cell cycle. According PubMed:16924674 is almost exclusively localized in the nucleus and appears in numerous small foci and a small number of larger foci whereas a centrosomal location has not been detected.,subunit:Interacts with PRKDC and PARP1. Interacts with XRCC5; the interaction is dependent on nucleic acids. Interacts with XRCC6; the interaction is dependent on nucleic acids. Interacts with EZH2, HDAC1, HDAC2, SUZ12, YY1, LIG3 and LIG4.,tissue specificity:Widely expressed; the highest levels are in the kidney, skeletal muscle, liver, heart and spleen; also detected in pancreas, lung, placenta, brain, leukocytes, colon, small intestine, ovary, testis, prostate and thymus., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |
| Material Safety Data Sheet | |
| English_US |
|
| Danish |
|
| Dutch |
|
| English_UK |
|
| French |
|
| German |
|
| Spanish |
|
| Norwegian |
|
| Portuguese |
|
| Finnish |
|
| Swedish |
|
| Polish |
|