ITT4253
ITT4253
- Catalog: ITT4253
- Gene/Protein: ATP2A1
- Product Description: Immunotag™ SERCA1 Polyclonal Antibody
385.0000
Price in reward points: 385
| Antibody Specification | |
| Datasheet | 
|
| Target Protein | SERCA1 |
| Clonality | Polyclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,IHC-p,ELISA |
| Recommended Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Mouse,Rat |
| Host Species | Rabbit |
| Immunogen | Synthesized peptide derived from SERCA1, at AA range: 520-600 |
| Specificity | SERCA1 Polyclonal Antibody detects endogenous levels of SERCA1 protein. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Gene Name | ATP2A1 |
| Accession No. | O14983 Q8R429 Q64578 |
| Alternate Names | ATP2A1; Sarcoplasmic/endoplasmic reticulum calcium ATPase 1; SERCA1; SR Ca(2+)-ATPase 1; Calcium pump 1; Calcium-transporting ATPase sarcoplasmic reticulum type; fast twitch skeletal muscle isoform; Endoplasmic reticulum class 1/2 Ca(2+) AT |
| Description | ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1(ATP2A1) Homo sapiens This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in muscular excitation and contraction. Mutations in this gene cause some autosomal recessive forms of Brody disease, characterized by increasing impairment of muscular relaxation during exercise. Alternative splicing results in three transcript variants encoding different isoforms. [provided by RefSeq, Oct 2013], |
| Cell Pathway/ Category | Calcium,Alzheimer's disease, |
| Protein Expression | Liver,Muscle,Skeletal muscle,Thymus,Tongue, |
| Subcellular Localization | mitochondrion,endoplasmic reticulum membrane,endoplasmic reticulum-Golgi intermediate compartment,integral component of plasma membrane,membrane,integral component of membrane,sarcoplasmic reticulum,platelet dense tubular network me |
| Protein Function | catalytic activity:ATP + H(2)O + Ca(2+)(Cis) = ADP + phosphate + Ca(2+)(Trans).,developmental stage:Isoform SERCA1A accounts for more than 99% of SERCA1 isoforms expressed in adult, while isoform SERCA1B predominates in neo-natal fibers.,disease:Defects in ATP2A1 are the cause of Brody disease (BD) [MIM:601003]. BD is an autosomal recessive myopathy characterized by increasing impairment of relaxation of fast twist skeletal muscle during exercise.,enzyme regulation:Reversibly inhibited by phospholamban (PLN) at low calcium concentrations. Dephosphorylated PLN decreases the apparent affinity of the ATPase for calcium. This inhibition is regulated by the phosphorylation of PLN.,function:This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.,induction:Increased contractile activity leads to decrease SERCA1 expression, while decreased contractile activity leads to increase of SERCA1 expression.,similarity:Belongs to the cation transport ATPase (P-type) family.,similarity:Belongs to the cation transport ATPase (P-type) family. Type IIA subfamily.,subunit:Associated with sarcolipin (SLN) and phospholamban (PLN).,tissue specificity:Skeletal muscle, fast twitch muscle (type II) fibers., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |
| Material Safety Data Sheet | |
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