ITT4374
ITT4374
- Catalog: ITT4374
- Gene/Protein: SP100
- Product Description: Immunotag™ SP-100 Polyclonal Antibody
385.0000
Price in reward points: 385
| Antibody Specification | |
| Datasheet | 
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| Target Protein | SP100 |
| Clonality | Polyclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,IHC-p,ELISA |
| Recommended Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human |
| Host Species | Rabbit |
| Immunogen | Synthesized peptide derived from SP-100 . at AA range: 250-330 |
| Specificity | SP-100 Polyclonal Antibody detects endogenous levels of SP-100 protein. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Gene Name | SP100 |
| Accession No. | P23497 O35892 |
| Alternate Names | SP100; Nuclear autoantigen Sp-100; Lysp100b; Nuclear dot-associated Sp100 protein; Speckled 100 kDa |
| Description | SP100 nuclear antigen(SP100) Homo sapiens This gene encodes a subnuclear organelle and major component of the PML (promyelocytic leukemia)-SP100 nuclear bodies. PML and SP100 are covalently modified by the SUMO-1 modifier, which is considered crucial to nuclear body interactions. The encoded protein binds heterochromatin proteins and is thought to play a role in tumorigenesis, immunity, and gene regulation. Alternatively spliced variants have been identified for this gene; one of which encodes a high-mobility group protein. [provided by RefSeq, Aug 2011], |
| Protein Expression | Cervix carcinoma,Epithelium,Kidney,Liver,Lymphoma,Mammary cancer,Placenta,Salivary |
| Subcellular Localization | nuclear chromosome, telomeric region,nucleus,nucleoplasm,nucleolus,cytoplasm,PML body,Mre11 complex,nuclear periphery, |
| Protein Function | disease:This antigen is recognized by autoantibodies from patients with primary biliary cirrhosis (PBC).,domain:Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.,domain:The HSR domain is important for the nuclear body targeting as well as for the dimerization.,function:May play a role in the control of gene expression.,induction:By interferon.,miscellaneous:The major isoform Sp100-A, has a calculated MW of 54 kDa, but exhibits aberrant electrophoretic mobilities, with an apparent MW OF 100 kDa.,PTM:Phosphorylated.,PTM:Sumoylated. Sumoylation depends on a functional nuclear localization signal but is not necessary for nuclear import or nuclear body targeting.,similarity:Contains 1 HSR domain.,similarity:Contains 1 SAND domain.,similarity:Contains 2 HMG box DNA-binding domains.,subcellular location:Found in the nuclear body, also known as nuclear domain 10 (ND10), PML oncogenic domain (POD), nuclear dots (ND) and KR body. The nuclear body is a nucleoplasmic structure of punctate shape, which varies in size and number. Induction by interferon and may be cell cycle stages modulate the subnuclear localization of the isoforms.,subunit:Homodimer. Splice variants heterodimerize. Interacts with members of the HP1 family of nonhistone chromosomal protein, such as CBX5 and CBX3 via the PxVxL motif. Interacts with Epstein-Barr virus EBNA-LP.,tissue specificity:Widely expressed. Sp100-B is expressed only in spleen, tonsil, thymus, mature B-cell line and some T-cell line, but not in brain, liver, muscle or non-lymphoid cell lines., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |
| Material Safety Data Sheet | |
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